ACP Antibodies

Antibodies targeting APC (Adenomatous Polyposis Coli) are commonly used in research and diagnostics to detect and study the expression of the APC protein. The APC gene is a tumor suppressor gene associated with the development of colorectal cancer and other cancers. The APC protein plays a crucial role in regulating cell proliferation and preventing the formation of tumors.

Here are some common applications of APC antibodies in research:

Immunohistochemistry (IHC): APC antibodies can be used in IHC to visualize the presence and localization of the APC protein in tissue sections. This is often done to study the expression patterns in normal and cancerous tissues.

Western Blotting: APC antibodies are used in Western blot analysis to detect the presence of the APC protein in cell lysates. This technique helps researchers assess protein expression levels and investigate molecular weight variants.

Immunofluorescence (IF): Immunofluorescence with APC antibodies allows for the visualization of APC protein in cells. This technique is useful for studying the subcellular localization of APC and its dynamics.

Flow Cytometry: Flow cytometry with APC antibodies can be used to analyze the expression of APC on the surface of cells. This is particularly relevant for studying immune cells.

Research on Cancer Biology: APC is a key player in the Wnt signaling pathway and is frequently mutated in colorectal cancers. Antibodies targeting APC are valuable tools for researchers studying cancer biology and mechanisms involved in tumor formation.

When working with antibodies, it's crucial to choose antibodies validated for specific applications and to follow recommended protocols for sample preparation and analysis. Researchers often obtain antibodies from reputable suppliers or generate them in-house, and they should ensure that the chosen antibody meets the requirements of their experimental setup.