PRDX3 Antibodies

PRDX3 (Peroxiredoxin 3) is a mitochondrial antioxidant enzyme belonging to the peroxiredoxin family, a group of thiol-specific antioxidants that play a crucial role in cellular redox regulation. PRDX3 specifically functions within the mitochondria, where it catalyzes the reduction of hydrogen peroxide (H₂O₂) to water, utilizing thioredoxin as an electron donor. 

Structure and Function

PRDX3 is a homodimeric protein composed of two identical subunits that form a functional dodecamer. Each monomer contains a conserved cysteine residue (Cys-108), which undergoes oxidation during the catalytic cycle. This cysteine residue is oxidized to sulfenic acid (Cys-SOH) by hydrogen peroxide, forming a disulfide bond with the resolving cysteine from another monomer. The disulfide bond is subsequently reduced by thioredoxin, regenerating the active enzyme. PRDX3 operates through a typical 2-Cys peroxiredoxin mechanism, which is critical for its antioxidant activity in the mitochondria.

PRDX3 Antibody Applications

PRDX3 antibodies are used extensively in research to study mitochondrial biology, oxidative stress, and redox signaling pathways. The antibodies can detect both the monomeric and dimeric forms of PRDX3 in western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) applications. These antibodies are critical for understanding PRDX3's role in disease models, as well as for exploring its potential as a therapeutic target. Additionally, PRDX3 antibodies can help delineate its expression patterns across different tissues and cells, particularly in oxidative stress-related studies.

Key Research Applications of PRDX3 Antibody:

• Western Blotting (WB): Detects the expression levels and post-translational modifications of PRDX3 in mitochondrial extracts.
• Immunohistochemistry (IHC): Enables localization of PRDX3 in tissue sections, facilitating studies on mitochondrial redox regulation in normal and disease states.
• Immunofluorescence (IF): Visualizes PRDX3 distribution within the mitochondria, aiding in studies related to oxidative stress.
• Flow Cytometry (FC): Quantifies PRDX3 expression in different cell populations under various redox conditions.

PRDX3 antibodies are indispensable tools for the investigation of mitochondrial function, redox homeostasis, and oxidative stress-related diseases. Through specific detection of PRDX3 in various experimental settings, these antibodies contribute to a deeper understanding of the enzyme’s role in maintaining mitochondrial integrity and its involvement in pathological conditions, including cancer, neurodegenerative diseases, and metabolic disorders.