AffiAB® Anti-PRDX6 Antibody [JM11-13]

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CAT# AFG-HB-3227
Size: 100 μL

496.50 496.5 USD 496.50

496.50

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AffiAB® Anti-PRDX6 Antibody [JM11-13]

The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis. The human PRX IV gene is abundantly expressed in many tissues. PRX IV exists as a precursor protein, which is only detected in testis, and a processed secreted form. PRX V also exists as two forms, designated long and short. Like PRX IV, the long form of PRX V is highly expressed in testis. The short form of PRX V is more widely expressed, with high expression in liver, kidney, heart and lung. PRX VI, a 1-Cys peroxiredoxin (also known as antioxidant protein 2 or AOP2) , is highly expressed in most tissues, particularly in epithelial cells. Localized to the cell cytosol, PRX VI functions independently of other peroxiredoxins and antioxidant proteins, specializing in antioxidant defense, lung phospholipid metabolism and protection of keratinocytes from cell death induced by reactive oxygen species.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: P30041 Human; SwissProt: O08709 Mouse; SwissProt: O35244 Rat

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

JM11-13

KO/KD

N/A

Species reactivity

Human

Tested applications

WB, IP, IF-Cell, IF-Tissue

Predicted species reactivity

Mouse, Rat

Immunogen

Synthetic peptide within Human PRDX6 aa 175-224 / 224.

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

Cardiovascular

Recommended dilutions

WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IP: 1:10-1:50

Molecular Weight

Predicted band size: 25 kDa

Subcellular location

Cytoplasm. Lysosome.

Positive control

HepG2 cell lysate, Hela cell lysate.