LRRC Antibodies
LRRC Antibodies are developed to specifically detect and bind to proteins that contain Leucine-Rich Repeat (LRR) domains, known as Leucine-Rich Repeat-Containing (LRRC) proteins. The LRR motif is a conserved structural domain found in various proteins involved in processes such as cell signaling, immune responses, protein-protein interactions, and cell adhesion.
Content of LRRC Antibody
LRRC Antibodies are produced by immunizing animals such as rabbits or mice with peptides or recombinant protein fragments containing LRR domains. These antibodies can be:
- Polyclonal antibodies: Recognize multiple epitopes across the LRRC protein, offering robust detection in various experimental assays.
- Monoclonal antibodies: Bind to a specific epitope within the LRR domain, providing high specificity and reproducibility for consistent experimental results.
Application of LRRC Antibody
LRRC Antibodies are widely used in cell signaling research, neurobiology, immunology, and cancer studies to investigate the functions of LRRC proteins and their roles in disease processes. Common applications include:
- Western Blotting: LRRC Antibodies are used in Western blot assays to detect the expression of LRRC proteins in cell or tissue lysates. This technique helps quantify the levels of LRRC proteins in different conditions, such as cancer, neurodegenerative diseases, or immune responses. For example, LRRC8 (a component of the volume-regulated anion channel) and LRRC4 (NGL-2, involved in synaptic adhesion) are studied in various contexts using Western blot.
- Immunohistochemistry (IHC): In IHC, LRRC Antibodies are applied to tissue sections to localize LRRC protein expression. This application is important for visualizing the spatial distribution of LRRC proteins in tissues such as the brain, immune organs, or tumor samples. For instance, LRRC4/NGL-2 is of particular interest in neurodevelopmental and psychiatric research, where its expression in brain tissues can provide insights into synaptic function and neurodevelopment.
- Immunofluorescence (IF): LRRC Antibodies conjugated to fluorophores are used in immunofluorescence assays to visualize the localization of LRRC proteins within cells. This is particularly useful for studying intracellular trafficking, protein localization, and cell signaling processes. For example, LRRC8, which forms part of ion channels, can be visualized in the cell membrane, and its role in osmotic regulation can be studied using IF.
LRRC Antibodies are powerful tools in biomedical research, enabling the study of Leucine-Rich Repeat-Containing (LRRC) proteins, which are involved in crucial biological processes such as cell signaling, immune regulation, neurodevelopment, and cancer progression. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to investigate the expression, localization, and function of LRRC proteins.
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