CEP Antibodies
CEP (Centrosomal Proteins) antibodies specifically target centrosomal proteins, which are integral to the function and structure of the centrosome, the microtubule-organizing center (MTOC) in eukaryotic cells. Centrosomal proteins, such as CEP135, CEP164, and others, are essential for various cellular processes, including centrosome duplication, cell cycle regulation, spindle formation, and ciliogenesis. Abnormalities in centrosomal proteins can lead to defects in cell division, contributing to disorders such as cancer, microcephaly, and ciliopathies. CEP antibodies are vital tools in research focused on the molecular mechanisms of these processes.
Content of CEP Antibodies
CEP antibodies are available in monoclonal and polyclonal forms, designed to detect specific centrosomal proteins in cells and tissues. These antibodies are used in several key assays including Western blotting, immunoprecipitation, immunofluorescence (IF), and immunohistochemistry (IHC) to investigate the localization, expression, and function of CEP proteins. Different CEP antibodies target various components, such as:
- CEP135: Critical for centrosome structure and centriole elongation. It is involved in the recruitment of microtubule nucleation factors during mitosis.
- CEP164: Required for ciliogenesis and proper functioning of the centrosome during cell cycle progression.
- CEP152: Plays a role in centriole duplication, contributing to the formation of two centrosomes from one during the cell cycle.
Application of CEP Antibodies
- Cell Cycle and Mitosis Research: CEP antibodies are instrumental in studying the centrosome’s role in cell division. Centrosomes are crucial for forming the mitotic spindle, which ensures accurate segregation of chromosomes during mitosis.
- Ciliopathies and Developmental Disorders: Ciliopathies are genetic disorders caused by defects in cilia formation or function. CEP164, for instance, is essential for ciliogenesis, and antibodies against it are used to study cilia-related defects. Mutations in centrosomal proteins like CEP164 can lead to disorders like nephronophthisis and Meckel-Gruber syndrome.
- Cancer Research: Centrosome amplification, where cells have more than two centrosomes, is frequently observed in cancer. CEP antibodies, such as those targeting CEP152, are used to study centrosome amplification and its contribution to tumorigenesis.
- Immunofluorescence (IF) and Immunohistochemistry (IHC): CEP antibodies are widely used in IF and IHC to visualize the localization of centrosomal proteins in cells and tissues. These techniques help researchers analyze the structural integrity of centrosomes, study centrosome-related diseases, and identify abnormal centrosome numbers in cancerous tissues.
CEP antibodies serve as powerful tools for understanding the biological and pathological roles of centrosomes in cell division, ciliogenesis, and disease states such as cancer and ciliopathies.
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