CASP8 Antibodies
CASP8 antibodies are specialized immunoglobulins that target Caspase-8 (CASP8), a crucial initiator caspase in the extrinsic apoptotic pathway. Caspase-8 is activated in response to signals from death receptors such as Fas (CD95) and TNF-related apoptosis-inducing ligand (TRAIL) receptors. Upon activation, CASP8 triggers the downstream apoptotic cascade, leading to programmed cell death.
Content
Caspase-8 (CASP8) is a cysteine-aspartic protease that plays a pivotal role in mediating extrinsic apoptosis. Upon ligand binding to death receptors (e.g., Fas, TRAIL-R), CASP8 is recruited to the death-inducing signaling complex (DISC) via adaptor proteins like FADD (Fas-Associated protein with Death Domain). Within the DISC, CASP8 undergoes dimerization and autocleavage, becoming activated and initiating the downstream cleavage of effector caspases (e.g., caspase-3 and caspase-7), leading to apoptosis.
Applications
- Western Blotting: CASP8 antibodies are widely used in Western blot assays to detect and quantify full-length and cleaved (active) forms of Caspase-8. This helps researchers monitor the activation of CASP8 under different experimental conditions, such as during cell stress, immune activation, or drug treatment.
- Immunohistochemistry (IHC): CASP8 antibodies are employed in IHC to visualize the expression and localization of CASP8 within tissue samples. This application is useful for studying the role of CASP8-mediated apoptosis in various tissues, including tumor biopsies, and understanding its involvement in disease progression.
- Flow Cytometry: Researchers use CASP8 antibodies in flow cytometric assays to analyze the expression of CASP8 in individual cells. Flow cytometry enables the detection of both the inactive (pro-caspase-8) and active forms of CASP8, providing insights into apoptosis regulation at a single-cell level.
CASP8 antibodies are indispensable tools in apoptosis research, allowing for the precise detection and analysis of caspase activation and function. Their use in various experimental techniques provides critical insights into the mechanisms of cell death and survival, aiding in the development of therapeutic strategies for conditions characterized by defective apoptotic pathways, such as cancer, autoimmune disorders, and neurodegenerative diseases.
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