RAB Antibodies

RAB antibodies are designed to detect and target RAB proteins, a large family of small GTPases belonging to the Ras superfamily. RAB GTPases play a crucial role in the regulation of intracellular vesicle trafficking, which includes vesicle formation, movement, and fusion with target membranes. 

Applications of RAB Antibodies

• Western Blotting (WB): RAB antibodies are commonly used in western blot analysis to detect the expression levels of RAB proteins in cell or tissue lysates. This application helps study changes in RAB expression in different physiological or pathological conditions, such as during cancer progression or neurodegenerative diseases.
• Immunoprecipitation (IP): RAB antibodies can immunoprecipitate RAB proteins, allowing for the study of RAB interactions with effector proteins and GTPase-activating proteins (GAPs). This is particularly useful in understanding how RAB proteins regulate specific vesicular trafficking events.
• Immunohistochemistry (IHC): These antibodies are used to localize RAB proteins in tissue sections, providing insights into their spatial distribution in various organs, especially in the context of diseases like cancer or neurodegeneration.

RAB Antibodies in Disease Research

• Cancer: Dysregulation of RAB proteins, such as RAB25 and RAB7, has been linked to cancer progression and metastasis. RAB proteins regulate intracellular trafficking pathways that influence cell proliferation, migration, and invasion. RAB antibodies are used to investigate the role of RAB proteins in oncogenic signaling pathways, tumor cell migration, and metastasis formation.
• Neurodegenerative Diseases: RAB proteins are involved in neuronal vesicle trafficking and autophagic processes. Dysfunctions in proteins such as RAB7 and RAB3 have been implicated in neurodegenerative disorders like Parkinson’s disease, Alzheimer’s disease, and Charcot-Marie-Tooth disease. RAB antibodies help explore how abnormal RAB signaling contributes to neurodegeneration and defective neuronal trafficking.
• Immunological Disorders: RAB27 plays a critical role in the exocytosis of cytotoxic granules in immune cells, such as cytotoxic T cells and natural killer (NK) cells. Mutations in RAB27A cause Griscelli syndrome, a rare immunodeficiency disorder. RAB antibodies are used to study immune cell dysfunction and vesicular transport defects in such conditions.

RAB antibodies are essential tools for investigating the role of RAB GTPases in intracellular vesicular trafficking and membrane dynamics. Through their use in techniques such as western blotting, immunohistochemistry, and immunoprecipitation, these antibodies enable researchers to explore the regulation of RAB proteins in normal physiology and disease states.