HOX Antibodies
HOX antibodies are designed to target proteins from the Homeobox (HOX) gene family, which plays a crucial role in regulating the development of the anterior-posterior axis and segmental identity in multicellular organisms. The HOX family of transcription factors is essential for proper cell differentiation and organogenesis, making these antibodies valuable in developmental biology and cancer research.
Content of HOX Antibodies
HOX antibodies are developed to specifically recognize HOX proteins based on their unique conserved domains.
Key specifications:
- Immunogen: Synthetic peptides or recombinant proteins corresponding to the conserved or unique regions of specific HOX proteins.
- Host: Commonly raised in rabbits or mice.
- Applications: Suitable for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), chromatin immunoprecipitation (ChIP), and flow cytometry (FC).
- Reactivity: HOX antibodies are commonly reactive with human, mouse, and rat proteins, though some may cross-react with other species.
Application of HOX Antibodies
HOX antibodies are extensively applied in the fields of developmental biology, oncology, and molecular genetics. Their applications include:
- Western Blotting (WB): HOX antibodies are used to detect and quantify specific HOX proteins in tissue lysates or cell extracts. This allows researchers to analyze the expression patterns of HOX genes during different stages of development or in various tissues.
- Immunohistochemistry (IHC): By applying HOX antibodies to tissue sections, researchers can study the spatial expression patterns of HOX proteins. This is particularly valuable for mapping developmental processes in embryonic tissues and for examining abnormal expression in cancerous tissues.
- Chromatin Immunoprecipitation (ChIP): HOX antibodies are used to investigate protein-DNA interactions by immunoprecipitating HOX proteins bound to their target DNA sequences. This application is critical for understanding the regulatory networks controlled by HOX genes and their influence on gene expression during development and differentiation.
HOX antibodies provide a powerful tool for understanding the role of HOX genes in embryogenesis, tissue specification, and their aberrant expression in diseases such as cancer, making them indispensable in both basic research and clinical diagnostics.
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