GPR Antibodies

G protein-coupled receptors (GPRs) are a large family of cell surface receptors that mediate signal transduction by interacting with G proteins. These receptors play a critical role in numerous physiological processes, including hormone response, neurotransmission, immune system regulation, and sensory perception. GPRs are involved in many signaling pathways and are major drug targets due to their involvement in various diseases such as cancer, cardiovascular diseases, and neurodegenerative disorders.

Content of GPR Antibodies

GPR antibodies are typically generated against specific epitopes within the extracellular or intracellular domains of GPR proteins. These antibodies can be monoclonal or polyclonal and are validated for the following applications:

• Western Blotting (WB): GPR antibodies are used to detect the expression of specific GPRs in cell and tissue lysates, providing insights into their regulation under physiological and pathological conditions.
• Immunoprecipitation (IP): These antibodies facilitate the isolation of GPR protein complexes, allowing researchers to study the interaction of GPRs with other proteins, ligands, or signaling molecules.
• Flow Cytometry: GPR antibodies are utilized in flow cytometry to quantify the surface expression of GPRs in single-cell populations, which is especially useful in characterizing cell subsets or studying receptor trafficking.
• ELISA: GPR-specific antibodies can be used in ELISA assays to measure the levels of GPR proteins in biological fluids or cell extracts, contributing to biomarker discovery and diagnostic applications.

Application of GPR Antibodies

• Drug Discovery and Development: GPRs are prominent drug targets, with approximately 40% of all currently marketed drugs acting on GPRs. GPR antibodies are used in screening assays to evaluate the binding of small molecules, peptides, or biologics to GPRs, enabling the discovery of new therapeutics.
• Cancer Research: Many GPRs are dysregulated in cancer, contributing to tumor growth, metastasis, and angiogenesis. GPR antibodies help in understanding the role of specific receptors, such as GPR56 in glioma or GPR54 in prostate cancer, providing avenues for targeted therapies.
• Cardiovascular Studies: GPRs play a crucial role in regulating heart rate, blood pressure, and vascular tone. GPR antibodies are employed in studying the role of receptors like GPR39 and GPR81 in cardiovascular physiology and their potential as therapeutic targets for hypertension and heart disease.
• Metabolic Disorders: GPRs regulate various metabolic processes, including glucose homeostasis, lipid metabolism, and energy balance. GPR antibodies are applied in metabolic research to study receptors like GPR120 and GPR40, which are potential targets for treating diabetes and obesity.

GPR antibodies are indispensable for understanding the diverse roles of GPRs in cellular signaling and disease, and they continue to play a critical role in therapeutic research and biomarker discovery.