AffiAB® Anti-Tyrosinase Antibody
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3, 4-dihydroxyphenylalanine) , also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5, 6-dihydroxyindole) to indole-5, 6 quinone.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P14679 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Tyrosinase aa 51-100 / 529.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:200-1:500; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200; FC:1:50-1:200
Molecular Weight
70 kDa
Subcellular location
Membrane.
Positive control
B16F1, Hela, human urethral melanoma tissue, A431.