AffiAB® Anti-Progesterone Receptor Antibody
The effects of progesterone are mediated by two functionally different isoforms of the progesterone receptor, PR-A and PR-B, which are transcribed from distinct, estrogen-inducible promoters within a single copy of the PR gene. The first 164 amino acids of PR-B are absent in PR-A. Progesterone-bound PR-A and PR-B have different transcription activation properties. Specifically, PR-B functions as a transcriptional activator in most cell and promoter contexts, while PR-A is transcriptionally inactive and functions as a strong ligand-dependent transdominant repressor of steroid hormone receptor transcriptional activity. An inhibitory domain (ID) , which maps to the amino terminus of the receptor, exists within both PR isoforms. Interestingly, the ID is functionally active only in PR-A and is necessary for steroid hormone transrepression by PR-A, suggesting that PR-A and PR-B may have different conformations in the cell.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P06401 Human; SwissProt: Q00175 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within N-terminal human Progesterone Receptor.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Cancer
Recommended dilutions
IF-Cell: 1:500-1:1, 000; IHC-P: 1:100-1:500
Molecular Weight
99 kDa
Subcellular location
Cytoplasm. Nucleus.
Positive control
A549, Hela, MCF-7, human uterus tissue, mouse testis tissue.