![AffiAB® Anti-LRP6 Antibody [A5E7]](/web/image/product.product/76853/image_1024/%5BAFG-HB-2340%5D%20AffiAB%C2%AE%20Anti-LRP6%20Antibody%20%5BA5E7%5D%20?unique=b83e256)
AffiAB® Anti-LRP6 Antibody [A5E7]
Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: O75581 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG2b
Clone
A5E7
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human LRP6 aa 100-300 / 1613.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein G affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000
; IHC-P: 1:50-1:200
Molecular Weight
180 kDa
Subcellular location
Endoplasmic reticulum, Cell membrane, Membrane raft.
Positive control
Hela cell lysates, human colon tissue.