AffiAB® Anti-IRAK-1 Antibody [JE60-03]

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CAT# AFG-HB-2149
Size: 100 μL

346.50 346.5 USD 346.50

346.50

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AffiAB® Anti-IRAK-1 Antibody [JE60-03]

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: P51617 Human; SwissProt: Q62406 Mouse

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

JE60-03

KO/KD

N/A

Species reactivity

Human, Mouse

Tested applications

WB, IHC-P

Predicted species reactivity

N/A

Immunogen

Recombinant protein within human IRAK-1 aa 201-500/712.

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

WB: 1:500-1:2, 000 ; IHC-P: 1:100-1:200

Molecular Weight

Predicted band size: 77 kDa

Subcellular location

Cytoplasm, Lipid droplet, Nucleus.

Positive control

Hela cell lysate, K562 cell lysate, 293 cell lysate, mouse brain tissue.