AffiAB® Anti-IRAK-1 Antibody

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CAT# AFG-HB-2148
Size: 100 μL

283.50 283.5 USD 283.50

283.50

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AffiAB® Anti-IRAK-1 Antibody

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Antibody type

Rabbit polyclonal Antibody

Uniprot ID

SwissProt: P51617 Human; SwissProt: Q62406 Mouse

Recombinant

NO

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

N/A

KO/KD

N/A

Species reactivity

Human, Mouse

Tested applications

WB, IHC-P, IF-Cell

Predicted species reactivity

N/A

Immunogen

Recombinant protein within human IRAK-1 aa 1-250.

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

PBS (pH7.4) , 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2.17 mg/ml

Purity

Immunogen affinity purified.

Signal pathway

N/A

Recommended dilutions

WB: 1:2, 000; IHC-P: 1:400-1:800

Molecular Weight

Predicted band size: 77 kDa

Subcellular location

Cytoplasm, Nucleus.

Positive control

293T cell lysates, rat brain tissue, human breast tissue, mouse large intestine tissue, Hela, PC-3M.