AffiAB® Anti-EEA1 Antibody [B0-A8] - Early Endosome Marker

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CAT# AFG-HB-1276
Size: 100 μL

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AffiAB® Anti-EEA1 Antibody [B0-A8] - Early Endosome Marker

EEA1 localizes exclusively to early endosomes?and has an important role in endosomal trafficking. EEA1 binds directly to the phospholipid phosphatidylinositol 3-phosphate?through its C-terminalFYVE domain?and forms a homodimer?through a?coiled coil. EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as?N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo. Due to the proteins importance in vesicular trafficking, a number of intracellular bacteria prevent EEA1 recruitment to the vacuole.Mycobacterium tuberculosis?is known to inhibit the recruitment of EEA1 to the phagosomal membrane through CamKII. Legionella pneumophila?also prevents EEA1 recruitment through a currently unknown mechanism. [5] Interestingly, the related pathogen Legionella longbeachae?recruits EEA1 and appears to replicate within a modified early endosome.

Antibody type

Mouse monoclonal Antibody

Uniprot ID

SwissProt: Q15075 Human; SwissProt: Q8BL66 Mouse; SwissProt: A0A0G2K051 Rat

Recombinant

NO

Conjugation

Non-conjugated

Host

Mouse

Isotype

IgG1

Clone

B0-A8

KO/KD

N/A

Species reactivity

Human, Mouse, Rat

Tested applications

WB, IHC-P

Predicted species reactivity

N/A

Immunogen

Synthetic peptide of the N terminal residues of Human EEA1.

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 mg/mL.

Purity

Immunogen affinity purified.

Signal pathway

N/A

Recommended dilutions

WB: 1:2, 000-1:5, 000 ; IHC-P: 1:50-1:200

Molecular Weight

Predicted band size: 162 kDa

Subcellular location

Cytoplasm, early endosome membrane.

Positive control

293, human colon cancer tissue, mouse prostate tissue.