![AffiAB® Anti-Hu CD22 Biotin [MEM-01]](/web/image/product.product/82556/image_1024/%5BAFG-EXB-1057%5D%20AffiAB%C2%AE%20Anti-Hu%20CD22%20Biotin%20%5BMEM-01%5D?unique=d31c23b)
Overview
CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2, 6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Specificity:
The antibody MEM-01 reacts with an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes. The antibody MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope "E"; it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.
Antigen
CD22
Clone
MEM-01
Species Reactivity
Human, Non-human primates
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Raji Burkitt's lymphoma cell line
Application
FC (QC tested), IP, WB
Regulatory status
RUO
Concentration
1 mg/ml
Format
Biotin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide