![AffiAB® Anti-Notch1 APC [mN1A]](/web/image/product.product/79912/image_1024/%5BAFG-EXB-0942%5D%20AffiAB%C2%AE%20Anti-Notch1%20APC%20%5BmN1A%5D?unique=0daa593)
Overview
Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.
Specificity:
The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.
Antigen
Notch1
Clone
mN1A
Species Reactivity
Mouse, Human
Negative Species
Rat
Isotype
Mouse IgG1 kappa
Immunogen
GST fusion protein containing cdc10-NCR region of mouse Notch1
Application
FC (QC tested)
Regulatory status
RUO
Concentration
0.1 mg/ml
Format
APC
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide