![AffiAB® Anti-Hu IgE APC [4H10]](/web/image/product.product/79833/image_1024/%5BAFG-EXB-0863%5D%20AffiAB%C2%AE%20Anti-Hu%20IgE%20APC%20%5B4H10%5D?unique=0daa593)
Overview
Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
Specificity:
The mouse monoclonal antibody 4H10 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL) .
Antigen
IgE
Clone
4H10
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Purified human IgE.
Application
FC
Regulatory status
RUO
Concentration
0.1 mg/ml
Format
APC
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide