![AffiAB® Anti-Hu CD33 Purified [WM53]](/web/image/product.product/79441/image_1024/%5BAFG-EXB-0471%5D%20AffiAB%C2%AE%20Anti-Hu%20CD33%20Purified%20%5BWM53%5D?unique=d31c23b)
Overview
CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM) -containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
Specificity:
The mouse monoclonal antibody WM53 reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
Antigen
CD33
Clone
WM53
Species Reactivity
Non-human primates, Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Human AML cells
Application
FC (QC tested), IP, WB, IHC (F), ICC, MC (CyTOF)
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide