TNKS Antibodies

TNKS (Tankyrase) refers to a family of poly(ADP-ribose) polymerases, consisting mainly of TNKS1 (PARP5a) and TNKS2 (PARP5b). These enzymes play essential roles in various cellular processes, including telomere length regulation, Wnt/β-catenin signaling, and mitotic spindle formation. Tankyrases are involved in protein-protein interactions through their ankyrin repeat clusters, and they facilitate the poly(ADP-ribosyl)ation (PARylation) of substrate proteins.

Molecular Applications of TNKS Antibodies

• Wnt/β-Catenin Signaling: TNKS is a key regulator of the Wnt/β-catenin pathway by targeting Axin for degradation. TNKS antibodies allow researchers to study how TNKS modulates this signaling pathway, which is crucial in cancer development, particularly colorectal cancer.
• Telomere Length Regulation: TNKS interacts with TERF1 (telomeric repeat-binding factor 1) to regulate telomere length. TNKS antibodies can be used to investigate TNKS involvement in telomere maintenance, a critical aspect of cell aging and cancer.
• Autophagy and Mitophagy: Tankyrase enzymes play a role in cellular homeostasis by modulating autophagy and mitophagy. Studies using TNKS antibodies help in understanding how TNKS regulates these processes, particularly in the context of metabolic diseases and neurodegeneration.

Antibody Validation and Specificity

TNKS antibodies undergo rigorous validation to ensure their specificity and sensitivity across species, including humans, mice, and rats. Validation includes testing in multiple experimental setups, such as tissue lysates, recombinant protein overexpression, and knockdown/knockout models to confirm the absence of cross-reactivity with other PARP family members.

• Epitope Specificity: TNKS antibodies are developed to target highly conserved regions of the protein, ensuring broad application in studies involving wild-type and mutated forms of TNKS.
• Sensitivity: These antibodies are validated for detecting both endogenous and overexpressed levels of TNKS in various cell lines, making them suitable for basic research and therapeutic development.

TNKS antibodies are essential tools in understanding the diverse biological roles of tankyrases in cellular signaling, telomere biology, and cancer research. Their ability to target TNKS1 and TNKS2 specifically allows for detailed studies of these enzymes in various disease models.