TNFRSF10A Antibodies

TNFRSF10A antibodies are specialized immunoglobulins that target TNFRSF10A, also known as TRAIL receptor 1 (TRAIL-R1) or DR4 (Death Receptor 4). TNFRSF10A is a cell surface receptor that binds to the TNF-related apoptosis-inducing ligand (TRAIL), triggering the extrinsic pathway of apoptosis. Activation of this receptor by TRAIL can lead to programmed cell death, making TNFRSF10A a critical player in immune surveillance, cancer biology, and therapeutic strategies aimed at selectively inducing apoptosis in cancer cells. 

Content

TNFRSF10A (TRAIL-R1/DR4) is a member of the tumor necrosis factor receptor (TNFR) superfamily. It is primarily expressed on the surface of various cell types, including cancer cells, and acts as a receptor for TRAIL. Upon binding of TRAIL, TNFRSF10A forms a death-inducing signaling complex (DISC) by recruiting adaptor proteins such as FADD (Fas-Associated protein with Death Domain) and pro-caspase-8.

Applications

• Western Blotting: TNFRSF10A antibodies are commonly used to detect and quantify the expression of TNFRSF10A in cell lysates and tissue samples. This allows researchers to analyze the receptor's expression patterns and understand how its regulation changes in different conditions, such as cancer, immune responses, or drug treatment.
• Immunohistochemistry (IHC): Researchers use TNFRSF10A antibodies in IHC to visualize the localization and distribution of TNFRSF10A in tissue sections. This technique is especially useful for studying the receptor's expression in tumor tissues, where its presence may correlate with sensitivity to TRAIL-induced apoptosis and therapeutic outcomes.
• Flow Cytometry: TNFRSF10A antibodies are used in flow cytometry to analyze the surface expression of the receptor on live cells. This application is vital for assessing how TNFRSF10A expression varies across different cell types, including cancer cells, immune cells, and normal tissues, and for studying the receptor's role in mediating apoptosis.
• Immunoprecipitation: Using TNFRSF10A antibodies, researchers can isolate the receptor and its associated protein complexes from cell extracts. This enables the study of protein-protein interactions at the DISC, helping to elucidate the molecular mechanisms underlying TRAIL-R1-mediated apoptotic signaling.

TNFRSF10A antibodies are vital tools in apoptosis and cancer research, providing insights into the regulation of cell death and survival mechanisms. They are essential for exploring the therapeutic potential of targeting TRAIL-R1 in cancer and for understanding the broader role of the receptor in immune regulation and cellular homeostasis.