STK Antibodies

Function and Importance of STK Antibodies: STK antibodies are designed to specifically detect and study the expression, activity, and regulation of serine/threonine kinases. These antibodies are critical for exploring the role of STKs in signaling pathways, particularly in regulating cell cycle progression, apoptosis, metabolism, and stress responses.

Applications of STK antibodies include:

• Western Blot (WB): Detects STK proteins and their phosphorylated forms in cell or tissue lysates, providing insights into kinase expression and activation under different physiological or pathological conditions.
• Immunoprecipitation (IP): Facilitates the isolation of STK-containing complexes, enabling the study of protein-protein interactions and post-translational modifications associated with kinase signaling.
• Immunohistochemistry (IHC) and Immunofluorescence (IF): These techniques are used to visualize the localization of STK proteins within tissues and cells, particularly in signaling compartments like the cytoplasm, nucleus, and plasma membrane.

STK Antibody Applications in Research:

• Cancer Research: STK antibodies are widely used to investigate the role of serine/threonine kinases in cancer progression and to evaluate the effectiveness of kinase inhibitors as potential cancer therapies. Akt, mTOR, and AMPK antibodies are frequently used in studies of tumor metabolism and cell survival.
• Metabolic Disease Research: AMPK antibodies are critical for studying energy metabolism and the regulation of glucose and lipid homeostasis in metabolic diseases such as diabetes and obesity. These antibodies help researchers explore how AMPK activity is modulated in response to cellular energy levels.
• Cell Cycle and DNA Repair Studies: CHK1 and CHK2 antibodies are used to study cell cycle checkpoints and the DNA damage response. These antibodies help researchers understand how cells maintain genomic integrity and how defects in these pathways contribute to cancer development.

STK antibodies are essential tools for studying the roles of serine/threonine kinases in cellular signaling, metabolism, and disease. By targeting specific STKs, such as Akt, AMPK, mTOR, and CHK1, researchers can investigate how these kinases regulate key cellular processes, including cell growth, metabolism, and DNA repair. STK antibodies are particularly valuable in cancer, metabolic disorder, and neurodegenerative disease research, providing insights into the molecular mechanisms underlying these conditions.