SEPT Antibodies
Function and Importance of SEPT Antibodies: SEPT antibodies are used to detect septin proteins, which are involved in the formation of protein filaments that participate in a variety of cellular functions. Septins function as scaffolding proteins that organize other proteins into specific spatial configurations, particularly during cell division (cytokinesis). They also regulate membrane compartmentalization, contributing to processes such as membrane fusion and microtubule stabilization.
Applications of SEPT antibodies in research include:
- Western Blot (WB): Used to detect SEPT proteins in cellular or tissue lysates, helping researchers understand the expression and regulatory patterns of septins.
- Immunofluorescence (IF): Enables visualization of septin distribution and localization within the cell, particularly around the cytoskeleton and cell membrane.
- Immunoprecipitation (IP): SEPT antibodies can pull down SEPT protein complexes, which are important for studying protein-protein interactions involving septins.
- Flow Cytometry (FACS): Utilized for analyzing the presence of SEPT proteins on the cell surface or in intracellular compartments.
SEPT Antibody Applications in Research:
- Cell Cycle and Cytokinesis Studies: SEPT antibodies are frequently employed to study the septin ring structure that forms during cytokinesis. In dividing cells, septins form a contractile ring at the cleavage furrow, and antibodies against SEPT2, SEPT6, or SEPT7 are used to study this process.
- Cancer Research: Researchers use SEPT9 and other SEPT antibodies to explore the role of septins in tumor growth, metastasis, and cell motility. SEPT9 antibody, in particular, is utilized in epigenetic assays as a biomarker for early cancer detection.
- Neuroscience and Neurodegeneration: SEPT antibodies aid in the study of septin-associated protein aggregates in neurodegenerative diseases. SEPT4 and SEPT5 antibodies are key tools for investigating the role of septins in the formation of protein inclusions like Lewy bodies and their contribution to neural cell death.
SEPT antibodies are valuable tools in cell biology and disease research, providing critical insights into the functional roles of septins in diverse cellular processes such as cell division, membrane dynamics, and cytoskeletal arrangement. Their relevance in diseases like cancer, neurodegeneration, and infectious diseases makes SEPT antibodies essential in both basic and translational research settings.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|