PARK7 Antibodies
PARK7 antibodies are developed to specifically target the Parkinson protein 7 (PARK7), also known as DJ-1. PARK7 is a multifunctional protein involved in various cellular processes, including oxidative stress response, mitochondrial regulation, and neuroprotection. Mutations in the PARK7 gene are associated with early-onset Parkinson’s disease (PD), making PARK7 a critical protein for studying neurodegenerative disorders.
Content
PARK7 (DJ-1) functions as a molecular chaperone, protecting cells from oxidative stress and maintaining mitochondrial integrity. It plays a neuroprotective role by mitigating oxidative damage, regulating cell survival pathways, and modulating mitochondrial function. Loss of function or mutations in the PARK7 gene lead to increased vulnerability to oxidative stress, which contributes to the pathogenesis of Parkinson’s disease.
Application
PARK7 antibodies are used in a range of research techniques, including:
- Western Blotting (WB): For the detection and quantification of PARK7 protein in cell lysates and tissue extracts. This helps in assessing the expression levels and possible post-translational modifications of DJ-1.
- Immunohistochemistry (IHC): Employed to visualize the distribution and localization of PARK7 in tissue sections, particularly in brain samples. IHC is critical for studying the presence and role of PARK7 in both normal and diseased states, such as in PD-affected brain regions.
- Immunofluorescence (IF): Allows for the detailed observation of PARK7 at the cellular level, enabling the study of its subcellular localization and interactions under various experimental conditions, including oxidative stress models.
- Enzyme-Linked Immunosorbent Assay (ELISA): Used for quantifying PARK7 protein levels in different biological fluids, such as cerebrospinal fluid (CSF) and serum, which is valuable for biomarker research in neurodegenerative diseases.
- Immunoprecipitation (IP): Applied to isolate and analyze PARK7 from complex protein mixtures, facilitating the study of its interactions with other proteins and its functional behavior under stress conditions.
These applications make PARK7 antibodies essential tools for research into Parkinson’s disease and other neurodegenerative disorders.
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