NUDT Antibodies

NUDT (Nudix Hydrolase) proteins are a family of enzymes that catalyze the hydrolysis of nucleoside diphosphates linked to other moieties (X), such as nucleotides, nucleotide sugars, and coenzymes. This enzymatic activity plays a crucial role in maintaining the integrity of cellular nucleotide pools by eliminating damaged or oxidized nucleotides, which can otherwise be incorporated into DNA or RNA and cause mutations.

Applications of NUDT Antibodies

NUDT antibodies are indispensable in several experimental contexts, especially in the study of nucleotide metabolism, oxidative stress, and cancer research:

• Western Blotting: NUDT antibodies are commonly used to detect specific NUDT family members, such as NUDT1 or NUDT15, in cell or tissue lysates. This helps researchers assess changes in NUDT protein levels in response to oxidative stress, DNA damage, or chemotherapy treatment.
• Immunohistochemistry (IHC): NUDT antibodies can be applied to tissue sections to visualize the distribution and expression of NUDT proteins in various tissues. This is particularly useful for studying the role of NUDT proteins in oxidative damage repair within different organs, such as the brain, liver, or cancer tissues.
• Immunocytochemistry (ICC): In cultured cells, NUDT antibodies are used to study the subcellular localization of NUDT proteins, which are often localized to the cytoplasm, nucleus, or mitochondria. This application helps researchers understand how NUDT proteins respond to cellular stress and prevent the incorporation of oxidized nucleotides into nucleic acids.

Key Characteristics of NUDT Antibodies

• Isoform-Specificity: NUDT antibodies are designed to be specific to particular NUDT family members, such as NUDT1 or NUDT15, ensuring accurate detection of these proteins in complex biological samples. This specificity is crucial for distinguishing between different NUDT isoforms, which have distinct functions and roles in cellular processes.
• Cross-reactivity: Many NUDT antibodies exhibit cross-reactivity with homologous proteins across species, including humans, mice, and rats, enabling their use in a wide range of model organisms for biomedical research.
• Post-translational Modification Sensitivity: Some NUDT antibodies are engineered to detect specific post-translational modifications, such as phosphorylation, on NUDT proteins. This is particularly useful for studying how NUDT activity is regulated in response to cellular signaling or stress.

NUDT antibodies are critical tools in the study of nucleotide metabolism, oxidative stress responses, DNA damage repair, and chemotherapy drug metabolism. Their application in cancer, pharmacogenomics, and oxidative stress-related diseases provides valuable insights into the molecular mechanisms underlying disease progression and therapeutic responses.