KLK Antibodies

KLK Antibodies are designed to detect and bind to Kallikreins (KLKs), a family of serine proteases involved in various physiological processes such as blood pressure regulation, semen liquefaction, skin desquamation, and inflammation. Kallikreins, particularly KLK1 through KLK15, have garnered significant attention in medical research due to their roles in cancer, particularly in prostate, breast, ovarian, and pancreatic cancers, where they function as potential biomarkers for disease diagnosis and prognosis.

Content of KLK Antibody

KLK Antibodies are typically produced by immunizing animals (e.g., rabbits, mice) with KLK protein fragments or peptides, leading to the generation of antibodies that can specifically bind to KLK proteins. Depending on the application, antibodies can be:

• Polyclonal antibodies: These recognize multiple epitopes on the target KLK protein, providing robust detection across various experimental conditions.
• Monoclonal antibodies: These bind to a single, specific epitope, offering high specificity and consistency, which is crucial for diagnostic assays or targeted research.

Application of KLK Antibody

KLK Antibodies are widely used in cancer research, diagnostics, and various physiological studies. Their applications include:

• Enzyme-Linked Immunosorbent Assay (ELISA): KLK Antibodies are also used in ELISA kits to quantitatively measure KLK levels in biological fluids such as blood, serum, or urine. This is particularly useful in clinical diagnostics, where elevated levels of specific KLKs, such as KLK2 or KLK11, serve as potential biomarkers for detecting early-stage cancers or monitoring disease recurrence.
• Flow Cytometry: In flow cytometry, KLK Antibodies conjugated to fluorophores are used to detect KLK expression on the surface or inside cells. This application is essential for studying KLK-related signaling pathways in cancer cells or immune cells, helping to uncover the proteolytic roles of KLKs in cancer progression or immune modulation.
• Immunoprecipitation (IP): KLK Antibodies are used in immunoprecipitation assays to isolate KLK proteins from complex mixtures of proteins in cell lysates or tissue homogenates. This is important for studying protein-protein interactions involving KLKs, which are critical for understanding their regulatory roles in proteolytic cascades.
• Dermatology and Skin Research: KLK Antibodies are applied in dermatological research to study KLK-related processes such as skin desquamation, where KLK5 and KLK7 are key proteases involved in skin shedding and barrier function. Dysregulation of these KLKs is associated with skin disorders such as atopic dermatitis and psoriasis.

KLK Antibodies are crucial tools in cancer research, diagnostics, and understanding the physiological roles of Kallikreins. From their role in cancer biomarker discovery to their function in dermatological research and inflammation studies, KLK Antibodies offer valuable insights into Kallikrein-related processes.