KGP Antibodies

KGP Antibody is a highly specific tool designed to target and bind to the Kgp (gingipain K) protein, a lysine-specific cysteine proteinase produced by Porphyromonas gingivalis. This bacterial pathogen is a major etiological agent in periodontitis, a chronic inflammatory disease of the gums. Kgp is one of the key virulence factors of P. gingivalis, contributing to the degradation of host tissues and immune evasion.

Content of KGP Antibody

The KGP Antibody is produced by immunizing animals, such as rabbits or mice, with recombinant or purified fragments of the Kgp protein. This process generates polyclonal or monoclonal antibodies that specifically recognize epitopes on the Kgp protease.Common forms of KGP Antibody include:

• Polyclonal antibodies: These are generated by multiple clones of immune cells and recognize various epitopes on Kgp, providing a broad detection spectrum.
• Monoclonal antibodies: These recognize a single epitope, offering higher specificity and consistency, especially for diagnostic or therapeutic applications.

Application of KGP Antibody

KGP Antibodies play an important role in microbiology, immunology, and oral health research, where Porphyromonas gingivalis and its virulence factors are being studied for their contribution to periodontal diseases and systemic conditions like cardiovascular disease. Some specific applications include:

• Western Blotting: KGP Antibodies are used to detect the presence of Kgp in bacterial lysates, particularly to confirm the expression of the virulence factor in clinical isolates of P. gingivalis or in recombinant systems. This helps in understanding the levels of Kgp production under different conditions and the effect of mutations or inhibitors on Kgp synthesis.
• Immunohistochemistry (IHC): In studies investigating the histopathology of periodontal disease, KGP Antibodies are used to detect Kgp within gum tissues. By staining tissue sections, researchers can determine the localization and abundance of P. gingivalis and its proteases, providing insights into the progression of tissue damage and inflammation.
• Immunofluorescence (IF): KGP Antibodies, when conjugated to fluorophores, are used in immunofluorescence microscopy to visualize the presence of Kgp in bacterial cells or in infected host tissues. This application is valuable for studying bacterial-host interactions and determining how P. gingivalis invades tissues and evades immune responses.

KGP Antibodies are invaluable tools for studying the virulence mechanisms of Porphyromonas gingivalis, particularly the role of Kgp in periodontal disease. These antibodies are used across various applications, including Western blotting, immunohistochemistry, immunofluorescence, ELISA, and vaccine research. ​