KCNN Antibodies

KCNN Antibody is designed to target and bind to KCNN proteins, which are small-conductance calcium-activated potassium channels (SK channels). These channels play a crucial role in regulating the electrical activity of excitable cells by modulating membrane potential in response to intracellular calcium levels. KCNN proteins are encoded by the KCNN1, KCNN2, and KCNN3 genes, and are widely expressed in the brain, heart, and other tissues.

Content of KCNN Antibody

The KCNN Antibody is typically generated by immunizing animals with a specific peptide sequence derived from the KCNN protein, often from a conserved region shared among the isoforms. Polyclonal antibodies can recognize multiple epitopes, while monoclonal versions are specific to a single epitope. These antibodies are purified and validated for research purposes, including Western blotting, immunohistochemistry, and immunofluorescence.

Application of KCNN Antibody

The KCNN Antibody is essential for several applications across neuroscience, cardiovascular research, and smooth muscle physiology due to the broad functionality of KCNN channels. Specific applications include:

• Neuroscience Research: KCNN channels are critical in controlling neuronal excitability and firing patterns. The KCNN Antibody is used to study the distribution and functional roles of KCNN proteins in brain tissues, especially in neurons where they modulate afterhyperpolarization (AHP) phases of action potentials. It is widely employed in the characterization of SK channel subtypes in different brain regions using Western blot or immunohistochemistry.
• Cardiovascular Research: In the heart, KCNN channels help regulate cardiac pacemaking and atrial fibrillation. KCNN Antibodies can be applied to identify the presence and alterations of KCNN isoforms (particularly SK2 and SK3) in cardiac tissues. Immunofluorescence techniques help visualize their expression in specific heart structures, while Western blotting can assess the expression levels in pathological conditions such as heart failure.
• Smooth Muscle Physiology: KCNN channels in smooth muscle cells contribute to muscle relaxation and contraction in response to calcium signaling. The KCNN Antibody is used to map the expression and regulation of these channels in smooth muscle tissues of the gastrointestinal tract, bladder, and vasculature.

The KCNN Antibody is a valuable tool in research focusing on ion channel regulation, calcium signaling, and excitability in neurons, cardiac cells, and smooth muscles. By providing insights into the functional roles and expression patterns of KCNN channels, it supports a wide array of studies in neuroscience, cardiovascular physiology, and pathophysiology, contributing to a deeper understanding of the mechanisms underlying normal and disease states.