IFIT Antibodies
IFIT (Interferon-Induced Protein with Tetratricopeptide Repeats) antibodies are specific to the IFIT family of proteins, which are induced by interferons and play a key role in the antiviral response. The IFIT family, including IFIT1, IFIT2, and IFIT3, contributes to the inhibition of viral replication by recognizing non-self RNA, modulating protein synthesis, and interacting with viral components.
Content of IFIT Antibody
IFIT antibodies are produced by immunizing host animals with synthetic peptides or recombinant proteins that represent conserved regions of IFIT proteins. These antibodies are optimized for detecting specific members of the IFIT family and are validated for a range of experimental applications.
Key specifications:
- Immunogen: Synthetic peptides or recombinant proteins from IFIT1, IFIT2, IFIT3, or other IFIT family members.
- Host: Typically raised in rabbits or mice.
- Applications: Suitable for Western blotting (WB), immunohistochemistry (IHC), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA).
- Reactivity: IFIT antibodies are generally reactive with human, mouse, and rat IFIT proteins, with potential cross-reactivity with other species depending on antibody specificity.
Application of IFIT Antibody
IFIT antibodies are widely used in research focused on immune responses to viral infections, particularly in understanding interferon-stimulated pathways and the role of IFIT proteins in antiviral defense.
- Western Blotting (WB): IFIT antibodies are employed in Western blotting to detect and quantify the expression levels of IFIT proteins in cells or tissues. This application is important for investigating the upregulation of IFITs in response to viral infections or interferon treatment.
- Immunofluorescence (IF): IFIT antibodies conjugated to fluorescent markers are used to observe the intracellular localization of IFIT proteins. This application is key to studying how IFIT proteins dynamically respond to viral infection within cells.
- Enzyme-Linked Immunosorbent Assay (ELISA): IFIT antibodies are also used in ELISA to measure the concentration of IFIT proteins in biological samples, such as serum or cell supernatants, helping researchers assess immune responses to infections or interferon therapies.
The use of IFIT antibodies is critical for understanding how the innate immune system responds to viral threats and how interferon signaling regulates these responses. They are particularly useful in studies on antiviral immunity, interferon-stimulated gene expression, and immune system modulation in various diseases.
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