IARS Antibodies

IARS (Isoleucyl-tRNA Synthetase) is an essential enzyme that catalyzes the attachment of isoleucine to its corresponding tRNA during protein synthesis. IARS plays a crucial role in translation accuracy and protein synthesis in cells, ensuring the correct incorporation of isoleucine into polypeptides. 

Content of IARS Antibodies

IARS antibodies are generated to specifically detect the IARS protein. These antibodies are produced by immunizing animals with synthetic peptides or recombinant proteins derived from conserved or unique regions of the IARS enzyme.

Key specifications:

• Immunogen: Synthetic peptides or recombinant proteins from IARS.
• Host: Typically raised in rabbits or mice.
• Applications: Suitable for Western blotting (WB), immunohistochemistry (IHC), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assays (ELISA).
• Reactivity: Generally reactive with human, mouse, and rat IARS, with potential cross-reactivity with other species.

Application of IARS Antibodies

IARS antibodies are used in diverse fields, particularly in studies of translation, cancer research, and neurobiology. The primary applications include:

• Western Blotting (WB): IARS antibodies are widely used in WB to detect and quantify IARS protein expression in various tissues and cell lysates. This application is crucial for investigating the regulation of protein synthesis in normal and pathological states, such as in cancer or neurodegenerative disorders.
• Immunofluorescence (IF): IARS antibodies are used in IF to visualize the subcellular localization of IARS within cells. This application is essential for examining how IARS distribution changes in response to cellular stress, disease states, or during development.
• Enzyme-Linked Immunosorbent Assay (ELISA): IARS antibodies are applied in ELISA to quantify IARS protein levels in biological samples, aiding in studies related to translation regulation, cancer progression, and other diseases linked to protein synthesis.

IARS antibodies are valuable tools in the study of translation mechanisms, tRNA synthetase function, and their involvement in diseases like cancer, neurodevelopmental disorders, and metabolic diseases. By targeting IARS, researchers can gain insights into fundamental aspects of protein synthesis and its regulation under various physiological and pathological conditions.