HVEM Antibodies

HVEM (Herpesvirus Entry Mediator), also known as TNFRSF14 (Tumor Necrosis Factor Receptor Superfamily Member 14), is an immunoregulatory receptor that is part of the TNF receptor superfamily. It interacts with ligands such as BTLA (B and T Lymphocyte Attenuator), LIGHT, and CD160 to modulate both immune activation and inhibition.

Content of HVEM Antibodies

HVEM antibodies are designed to recognize specific epitopes on the HVEM protein. These antibodies are typically produced by immunizing host animals with synthetic peptides or recombinant proteins corresponding to extracellular or intracellular regions of the HVEM protein.

Key specifications:

• Immunogen: Synthetic peptides or recombinant proteins from HVEM.
• Host: Typically raised in rabbits, mice, or goats.
• Applications: Suitable for Western blotting (WB), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and enzyme-linked immunosorbent assays (ELISA).
• Reactivity: HVEM antibodies typically react with human HVEM but can also cross-react with mouse, rat, and other species depending on the antibody specificity.

Application of HVEM Antibodies

HVEM antibodies are widely used in immunological and oncological research, particularly for studying immune checkpoint pathways, viral entry mechanisms, and tumor immune evasion. Key applications include:

• Western Blotting (WB): HVEM antibodies are used to detect the presence and quantify HVEM expression in cell or tissue lysates. This helps in studying its role in immune signaling and its dysregulation in diseases like cancer or during viral infection.
• Immunohistochemistry (IHC): Using HVEM antibodies, researchers can analyze the expression and localization of HVEM in tissue samples, which is crucial for understanding its function in tumor microenvironments or lymphoid tissues.
• Flow Cytometry (FC): HVEM antibodies are often conjugated to fluorescent labels for flow cytometric analysis, allowing the quantification of HVEM expression on the surface of immune cells. This application is essential in characterizing immune cell populations and their interaction with tumor cells or viral pathogens.

HVEM antibodies are critical tools in immunology, helping to dissect the complex interactions between immune cells and tumor or pathogen-driven immune evasion mechanisms, and aiding in the development of immunotherapies targeting the HVEM signaling axis.