HTR Antibodies
HTR antibodies target serotonin receptors (5-HT receptors), which are G-protein-coupled receptors (GPCRs) responsible for mediating the effects of the neurotransmitter serotonin (5-hydroxytryptamine, or 5-HT). These receptors play critical roles in regulating mood, cognition, sleep, appetite, and various other physiological processes. The HTR family is divided into multiple subtypes (e.g., HTR1, HTR2, HTR3, etc.), each with distinct signaling pathways and tissue-specific expression patterns.
Content of HTR Antibody
HTR antibodies are designed to specifically recognize individual subtypes of serotonin receptors (e.g., HTR1A, HTR2A, HTR3A), targeting unique or conserved sequences within these receptor proteins.
Key specifications:
- Immunogen: Synthetic peptides or recombinant proteins from specific HTR subtypes (e.g., HTR1A, HTR2A, HTR3A).
- Host: Typically raised in rabbits or mice.
- Applications: Suitable for Western blotting (WB), immunohistochemistry (IHC), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA).
- Reactivity: Generally reactive with human, mouse, and rat serotonin receptors, with potential cross-reactivity depending on the receptor subtype.
Application of HTR Antibody
HTR antibodies are critical for investigating serotonin receptor biology, drug development, and the pathophysiology of psychiatric and neurological disorders. The applications include:
- Immunoprecipitation (IP): HTR antibodies facilitate the isolation of serotonin receptors from cell lysates, enabling the study of receptor-protein interactions. This is crucial for identifying signaling pathways associated with serotonin receptors and how they are modulated by various ligands or drugs.
- Immunofluorescence (IF): HTR antibodies conjugated with fluorescent tags are used to study the localization and trafficking of serotonin receptors within cells. This helps researchers understand how serotonin receptors are regulated and how they move to and from the cell membrane in response to different stimuli.
- Enzyme-Linked Immunosorbent Assay (ELISA): HTR antibodies are applied in ELISA assays to quantify serotonin receptor levels in biological samples, helping to measure receptor density changes in response to pharmacological treatments or in disease states.
HTR antibodies are essential tools in neurobiology and pharmacology for exploring the role of serotonin receptors in mental health, the development of antidepressants and antipsychotic drugs, and their involvement in conditions like depression, schizophrenia, and anxiety disorders.
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