HRP Antibodies

Horseradish Peroxidase (HRP) antibodies are conjugated antibodies that are chemically linked to horseradish peroxidase, an enzyme widely used in biochemical assays to catalyze the oxidation of substrates, producing a colorimetric, chemiluminescent, or fluorescent signal. HRP is frequently used as a labeling agent in secondary antibodies due to its stability, cost-effectiveness, and ability to generate strong signals.

Content of HRP Antibodies

HRP antibodies are typically secondary antibodies conjugated with the HRP enzyme. These antibodies are produced by immunizing a host species (commonly rabbit, goat, or mouse) with an antigen from another species, followed by chemical conjugation of the antibody with the HRP enzyme. 

Key specifications:

  • Immunogen: Antigens from species-specific immunoglobulin (e.g., anti-mouse IgG, anti-rabbit IgG).
  • Host: Typically raised in rabbits, goats, or mice.
  • Conjugation: Covalently linked to horseradish peroxidase (HRP) enzyme.
  • Applications: Suitable for Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), immunocytochemistry (ICC), and chemiluminescent assays.
  • Reactivity: Typically recognizes immunoglobulins from species such as human, mouse, rabbit, rat, etc.
Application of HRP Antibodies

HRP-conjugated antibodies are extensively used in immunoassays due to the enzyme's ability to produce a detectable signal when a substrate such as 3,3′,5,5′-tetramethylbenzidine (TMB) or enhanced chemiluminescent (ECL) reagents are added. The applications include:

  • Enzyme-Linked Immunosorbent Assay (ELISA): HRP antibodies are critical in ELISA, where they bind to the primary antibody or antigen on a solid surface. The addition of a substrate, such as TMB, results in a colorimetric change that quantifies the presence of the antigen or antibody being detected.
  • Immunohistochemistry (IHC): HRP-conjugated antibodies are used in IHC to detect the presence of specific proteins in tissue sections. HRP catalyzes a chromogenic substrate reaction, creating a localized color change that reveals the tissue distribution of the target antigen.
  • Immunocytochemistry (ICC): HRP antibodies can be used in ICC for detecting antigens within cells, allowing researchers to visualize protein localization using light microscopy after a substrate reaction has occurred.

The use of HRP-conjugated antibodies significantly enhances assay sensitivity and visualization, making them indispensable in molecular biology, diagnostics, and research. HRP antibodies provide robust detection and amplification of the target antigen, enabling researchers to achieve accurate results in various immunoassay platforms.


1,251.25 1251.25 USD
351.50 351.5 USD
351.50 351.5 USD
351.50 351.5 USD
AffiAB® Rabbit Anti-Ovalbumin Antibody-HRP labeled
CAT# AFG-DDA-8528
Size: 100 μL
351.50 351.5 USD
351.50 351.5 USD
351.50 351.5 USD
351.50 351.5 USD
351.50 351.5 USD
AffiAB® Rabbit Anti-Casein Antibody-HRP labeled
CAT# AFG-DDA-8401
Size: 100 μL
332.50 332.5 USD
AffiAB® Mouse Anti-His-tag Antibody-HRP labeled
CAT# AFG-DDA-8400
Size: 100 μL
332.50 332.5 USD
AffiAB® Rabbit Anti-HRP Antibody
CAT# AFG-DDA-8395
Size: 100 μL
332.50 332.5 USD
332.50 332.5 USD
AffiAB® Rabbit Anti-Myc-tag Antibody-HRP labeled
CAT# AFG-DDA-8335
Size: 100 μL
332.50 332.5 USD
AffiAB® Rabbit Anti-His-tag Antibody-HRP labeled
CAT# AFG-DDA-8326
Size: 100 μL
332.50 332.5 USD
AffiAB® Rabbit Anti-HA-tag Antibody-HRP labeled
CAT# AFG-DDA-8325
Size: 100 μL
332.50 332.5 USD
AffiAB® Rabbit Anti-FLAG-tag Antibody-HRP labeled
CAT# AFG-DDA-8320
Size: 100 μL
332.50 332.5 USD
AffiAB® Mouse Anti-GAPDH Antibody-HRP labeled
CAT# AFG-DDA-7457
Size: 100 μL
319.20 319.2 USD
319.20 319.2 USD
AffiAB® Rabbit Anti-HRP2 Antibody
CAT# AFG-DDA-4600
Size: 100 μL
300.20 300.2 USD
AffiAB® Rabbit Anti-PTHRP Antibody
CAT# AFG-DDA-4572
Size: 100 μL
300.20 300.2 USD
256.50 256.5 USD
AffiAB® Histidine-rich protein PFHRP-II Polyclonal Antibody
CAT# AFG-PAB-17177
Size: 100 µg
Conjugation: Unconjugated
533.50 533.5 USD
AffiAB® Streptavidin Poly-HRP40
CAT# AFG-ABX-002
Size: 200 µg
656.00 656.0 USD
AffiAB® Rabbit anti-PTHRP Antibody
CAT# AFG-YBP-6716
Size: 100 µL
457.00 457.0 USD
AffiAB® Rabbit anti-Ovalbumin Antibody-HRP labeled
CAT# AFG-YBP-5963
Size: 100 µL
457.00 457.0 USD
AffiAB® Rabbit anti-Myc-tag Antibody-HRP labeled
CAT# AFG-YBP-5470
Size: 100 µL
457.00 457.0 USD
457.00 457.0 USD
AffiAB® Rabbit anti-HRP2 Antibody
CAT# AFG-YBP-4317
Size: 100 µL
457.00 457.0 USD
AffiAB® Rabbit anti-HRP Antibody
CAT# AFG-YBP-4316
Size: 100 µL
457.00 457.0 USD
AffiAB® Rabbit anti-His-tag Antibody-HRP labeled
CAT# AFG-YBP-4020
Size: 100 µL
457.00 457.0 USD
AffiAB® Rabbit anti-HA-tag Antibody-HRP labeled
CAT# AFG-YBP-3935
Size: 100 µL
457.00 457.0 USD
AffiAB® Rabbit anti-FLAG-tag Antibody-HRP labeled
CAT# AFG-YBP-3327
Size: 100 µL
457.00 457.0 USD
AffiAB® Rabbit anti-Casein Antibody-HRP labeled
CAT# AFG-YBP-1355
Size: 100 µL
457.00 457.0 USD
457.00 457.0 USD
457.00 457.0 USD
AffiAB® Mouse anti-His-tag Antibody-HRP labeled
CAT# AFG-YBP-0122
Size: 100 µL
457.00 457.0 USD
457.00 457.0 USD
457.00 457.0 USD
457.00 457.0 USD
AffiAB® Mouse anti-GAPDH Antibody-HRP labeled
CAT# AFG-YBP-0091
Size: 100 µL
457.00 457.0 USD
457.00 457.0 USD
457.00 457.0 USD
457.00 457.0 USD
457.00 457.0 USD
AffiAB® Rabbit Anti-Human IgM HRP
CAT# AFG-YBS-0575
Size: 100 µL
130.00 130.0 USD
AffiAB® Rabbit Anti-Duck IgY (H&L) HRP
CAT# AFG-YBS-0437
Size: 100 µL
60.00 60.0 USD
AffiAB® Rabbit Anti-Chicken IgY (H&L) HRP
CAT# AFG-YBS-0389
Size: 100 µL
60.00 60.0 USD
AffiAB® Mouse Anti-Duck IgY (H&L) HRP
CAT# AFG-YBS-0215
Size: 100 µL
90.00 90.0 USD
AffiAB® Mouse Anti-Chicken IgY (H&L) HRP
CAT# AFG-YBS-0199
Size: 100 µL
90.00 90.0 USD
AffiAB® Goat Anti-Human IgM mu chain HRP
CAT# AFG-YBS-0122
Size: 100 µL
130.00 130.0 USD
AffiAB® Goat Anti-Chicken IgY (H&L) HRP
CAT# AFG-YBS-0001
Size: 100 µL
171.00 171.0 USD
AffiAB® HRP Conjugated Anti-6X His-Tag Antibody
CAT# AFG-HB-4193
Size: 100 μL
433.50 433.5 USD
611.15 611.15 USD
611.15 611.15 USD