HOMER Antibodies

HOMER proteins are a family of scaffolding proteins, primarily involved in synaptic function and signal transduction. HOMER antibodies target these proteins, aiding in the detection and analysis of their expression levels, localization, and functional roles in various tissues, particularly in the nervous system. HOMER proteins play a significant role in regulating intracellular calcium signaling pathways, particularly in their interactions with metabotropic glutamate receptors (mGluRs), ryanodine receptors, and inositol 1,4,5-trisphosphate (IP3) receptors.

Content of HOMER Antibody

The HOMER Antibody is a highly specific polyclonal or monoclonal antibody designed to detect different isoforms of HOMER proteins, such as HOMER1, HOMER2, and HOMER3. It is generated through immunization with synthetic peptides corresponding to the specific protein sequences in HOMER family members.

Key specifications:

• Immunogen: Synthetic peptides corresponding to conserved regions within HOMER proteins.
• Host: Typically raised in species such as rabbit or mouse.
• Applications: Suitable for use in Western blotting (WB), immunocytochemistry (ICC), immunohistochemistry (IHC), and immunoprecipitation (IP).
• Reactivity: Recognizes human, mouse, and rat HOMER proteins, with cross-reactivity in certain other species depending on the isoform.

Application of HOMER Antibody

HOMER Antibody is extensively used in neuroscience research, particularly in studying synaptic plasticity and neuronal signaling. Its application spans multiple research techniques:

• Western Blotting (WB): HOMER Antibody is used to detect and quantify HOMER protein isoforms in various tissues, such as brain homogenates, providing insights into their expression patterns during neuronal development or in response to synaptic activity.
• Immunohistochemistry (IHC): HOMER Antibody can be applied to fixed brain sections to visualize the spatial distribution of HOMER proteins in neurons. It is particularly useful in mapping synaptic regions and postsynaptic densities where HOMER proteins colocalize with receptors like mGluRs.
• Immunoprecipitation (IP): HOMER Antibody facilitates the immunoprecipitation of HOMER proteins, enabling the study of protein-protein interactions. It helps in identifying the complex formation of HOMER with other signaling molecules, including ionotropic and metabotropic receptors, crucial for synaptic communication.

By utilizing HOMER Antibody, researchers can investigate the role of HOMER proteins in various physiological and pathological contexts, such as synaptic plasticity, memory formation, and neurodegenerative diseases.