GSTA Antibodies

Glutathione S-transferase A (GSTA) is a family of enzymes involved in the detoxification process by catalyzing the conjugation of reduced glutathione to various hydrophobic and electrophilic compounds. GSTA proteins play a significant role in protecting cells from oxidative damage and the effects of xenobiotics. GSTA antibodies are used to detect and quantify these enzymes in various biological samples, providing insight into detoxification pathways and their regulation under different physiological and pathological conditions.

Content on GSTA Antibodies

GSTA is a key enzyme in Phase II metabolism and is involved in cellular protection against oxidative stress. GSTA antibodies are generated to target specific isoforms within the GSTA family, such as GSTA1, GSTA2, and GSTA3, each playing distinct roles in tissue-specific detoxification processes. These antibodies can be polyclonal or monoclonal, depending on their specificity and intended application.

Application of GSTA Antibodies

• Western Blotting: GSTA antibodies are commonly used in Western blot analysis to detect the expression levels of GSTA proteins in cell or tissue lysates. The differential expression of GSTA isoforms can be analyzed under various conditions such as oxidative stress or exposure to toxic compounds.
• Immunohistochemistry (IHC): In IHC, GSTA antibodies allow for the localization of GSTA proteins within tissue sections, providing valuable data on the spatial distribution of GSTA in healthy and diseased tissues, such as liver, kidney, and lung.
• Enzyme-linked Immunosorbent Assay (ELISA): GSTA antibodies are used in ELISA assays to quantify GSTA protein levels in serum, plasma, or tissue extracts, useful in toxicology and pharmacokinetics studies to monitor detoxification processes.
• Flow Cytometry: GSTA antibodies can be utilized in flow cytometry to study the expression of GSTA proteins at a single-cell level. This is particularly useful in investigating the role of GSTA in immune cells and its involvement in drug resistance mechanisms.
• Drug Development: GSTA antibodies are important in drug development studies, particularly in assessing the role of GSTA in drug metabolism and resistance. These studies can help identify compounds that modulate GSTA activity, leading to the development of drugs that can overcome detoxification-related resistance.

By employing GSTA antibodies, researchers gain insights into the enzyme’s involvement in detoxification pathways and its implications in health and disease.