GNMT Antibodies

Glycine N-methyltransferase (GNMT) is a key enzyme involved in methylation processes, primarily regulating the levels of S-adenosylmethionine (SAMe) in cells. GNMT catalyzes the conversion of glycine and SAMe to sarcosine, playing a critical role in methyl group metabolism and detoxification pathways. T

Content of GNMT Antibodies

GNMT antibodies are developed against epitopes of the GNMT protein, typically using recombinant or purified protein fragments. These antibodies can be monoclonal or polyclonal and are validated for multiple applications such as:

• Western blotting (WB): GNMT antibodies can be used to detect the presence and quantify the expression of GNMT in cell and tissue lysates through the identification of a specific band on a blot.
• Immunohistochemistry (IHC): In tissue sections, GNMT antibodies are applied to visualize the distribution and abundance of GNMT in situ, enabling the correlation of GNMT expression with tissue morphology and pathological states.
• Immunoprecipitation (IP): These antibodies facilitate the isolation of GNMT protein complexes, allowing for further study of protein-protein interactions and GNMT’s role in metabolic pathways.
• Enzyme-linked immunosorbent assay (ELISA): GNMT-specific antibodies are often incorporated into ELISA kits for quantifying GNMT levels in various biological samples such as serum, plasma, or tissue extracts.

Application of GNMT Antibodies

• Cancer Research: GNMT is known to play a dual role as a tumor suppressor gene in various cancers, including liver and prostate cancer. GNMT antibodies are essential in studying its expression patterns and correlating them with disease progression and prognosis.
• Liver Metabolism Studies: Given the enzyme's significant role in hepatic methylation processes, GNMT antibodies are frequently used to study liver function, particularly in conditions like non-alcoholic fatty liver disease (NAFLD) and cirrhosis.
• Epigenetic Regulation: Since GNMT impacts SAMe levels and DNA methylation, antibodies against GNMT are crucial for investigating the regulatory mechanisms of epigenetic changes in health and disease.
• Drug Development: GNMT is a potential therapeutic target in methylation disorders and cancers. Anti-GNMT antibodies are applied in drug screening assays to monitor the effectiveness of compounds that influence GNMT expression or function.

These technical applications highlight the versatility of GNMT antibodies in advancing the understanding of GNMT-related biological processes and their therapeutic potential.