FN1 Antibodies

FN1 antibodies are specialized antibodies designed to target and detect Fibronectin 1 (FN1), a high-molecular-weight glycoprotein that is part of the extracellular matrix (ECM). Fibronectin plays a crucial role in cell adhesion, migration, wound healing, and tissue repair. 

Content: FN1 antibodies can be used to target various forms and domains of fibronectin:

• Plasma Fibronectin: Soluble fibronectin found in the bloodstream, synthesized primarily by hepatocytes (liver cells). It plays a role in blood clotting, wound healing, and immune function. Antibodies against plasma fibronectin are used to study its role in hemostasis and its behavior under different pathological conditions.
• Modular Domains of Fibronectin: Fibronectin consists of various domains, including Type I, Type II, and Type III repeats, which mediate interactions with integrins, collagen, fibrin, and other ECM proteins. FN1 antibodies targeting specific domains are used to study fibronectin’s interactions with other molecules and its role in matrix assembly and cell signaling.

Applications: FN1 antibodies are widely used in various research and clinical applications, including:

• Enzyme-Linked Immunosorbent Assay (ELISA): To measure soluble fibronectin levels in blood, cell culture supernatants, or other biological samples. ELISA assays using FN1 antibodies are valuable for studying systemic changes in fibronectin expression, which can be indicative of disease states, including liver disease, cardiovascular disorders, and chronic inflammatory conditions.
• Cell Adhesion and Migration Assays: Fibronectin plays a key role in cell adhesion, a process crucial for tissue repair and development. FN1 antibodies can be used to block fibronectin-integrin interactions, allowing researchers to study the effects of disrupting cell adhesion and migration. This application is particularly relevant in cancer research, where the modulation of fibronectin can influence tumor cell behavior.
• Flow Cytometry: To analyze the expression of fibronectin on the surface of cells or in association with cell-secreted ECM. Flow cytometry allows researchers to study how fibronectin expression is regulated on a cellular level and can be used to evaluate the impact of different treatments on ECM composition.

FN1 antibodies are crucial tools for advancing our understanding of the extracellular matrix and its role in normal physiology and disease. They enable researchers to study how fibronectin contributes to tissue structure, cellular behavior, and pathological processes like cancer metastasis, wound healing, and fibrosis.