FADD Antibodies

FADD (Fas-Associated protein with Death Domain) antibodies are specific immunoglobulins developed to target and bind to the FADD protein, a crucial adaptor molecule in the apoptosis signaling pathway. FADD plays a key role in mediating cell death signals from death receptors, such as Fas (CD95) and TNF receptor 1 (TNFR1), by recruiting and activating downstream caspases. 

Content

FADD is an adaptor protein that contains a death domain (DD) and a death effector domain (DED). Upon activation of death receptors (e.g., Fas or TNFR1), FADD is recruited to the receptor complex, where it interacts with the intracellular death domains of these receptors. This recruitment facilitates the formation of the death-inducing signaling complex (DISC), leading to the activation of initiator caspases, such as caspase-8 and caspase-10, which then trigger the downstream apoptotic cascade. 

Applications

• Western Blotting: FADD antibodies are commonly used to detect and quantify FADD protein levels in cell and tissue lysates. Western blot analysis helps researchers study changes in FADD expression under different conditions, such as during immune activation, cancer progression, or drug treatment, providing insights into the regulation of apoptosis.
• Immunoprecipitation: FADD antibodies can be used to pull down FADD protein complexes from cell extracts, enabling the study of protein-protein interactions. This application is valuable for understanding how FADD interacts with other components of the DISC and identifying novel regulatory mechanisms that modulate the apoptotic pathway.
• Immunohistochemistry (IHC): Researchers use FADD antibodies in IHC to examine the localization and distribution of FADD within tissue samples. This is particularly useful for studying the role of FADD in different tissues, including tumors, and understanding how changes in FADD expression or localization may contribute to disease development.

FADD antibodies are essential tools for studying the intricate mechanisms of cell death and survival, providing critical insights into the regulation of apoptosis and the immune response. They enable researchers to explore the molecular basis of diseases characterized by dysregulated apoptosis, such as cancer and autoimmune disorders, and to develop potential therapeutic approaches to modulate these processes for clinical benefit.