EPAS Antibodies

EPAS (Endothelial PAS Domain Protein 1), also known as Hypoxia-Inducible Factor-2α (HIF-2α), is a transcription factor that plays a crucial role in the cellular response to hypoxia (low oxygen levels). It is a part of the HIF family, which is involved in regulating genes that control angiogenesis, metabolism, and cell survival under hypoxic conditions. 

Content on EPAS Antibodies

EPAS antibodies are used to detect EPAS protein expression in cells and tissues. These antibodies are valuable tools in molecular biology techniques such as Western blotting, immunoprecipitation, immunohistochemistry, and chromatin immunoprecipitation (ChIP). They enable the investigation of EPAS function in regulating gene expression under hypoxic conditions and are widely used to study the transcriptional networks activated by EPAS in response to low oxygen levels.

Applications of EPAS Antibodies

• Cancer Research: EPAS is frequently overexpressed in tumors and contributes to the adaptation of cancer cells to hypoxic conditions. It plays a significant role in promoting angiogenesis, cell survival, and tumor growth under low oxygen levels. EPAS antibodies are applied in cancer research to investigate how EPAS drives tumor progression and how targeting EPAS may serve as a therapeutic strategy.
• Kidney Function and Disease: EPAS is highly expressed in the kidneys, where it regulates genes involved in erythropoiesis (red blood cell production) and oxygen sensing. EPAS antibodies are used in kidney research to investigate its role in diseases such as chronic kidney disease (CKD) and renal cell carcinoma (RCC). 
• Transcriptional Regulation and Chromatin Studies: As a transcription factor, EPAS binds to hypoxia response elements (HREs) in the promoters of target genes to regulate their expression. EPAS antibodies are used in chromatin immunoprecipitation (ChIP) assays to identify EPAS-binding sites in the genome, providing insights into the transcriptional programs activated by hypoxia. 

In conclusion, EPAS antibodies are essential tools for studying the role of HIF-2α in hypoxia signaling, cancer progression, angiogenesis, and metabolic adaptation. They provide valuable insights into how EPAS regulates cellular responses to low oxygen and how its dysregulation contributes to diseases such as cancer, cardiovascular conditions, and pulmonary hypertension.