CCNK Antibodies

Cyclin K (CCNK) is a member of the cyclin family, which plays a key role in cell cycle regulation and transcriptional control. CCNK, in particular, interacts with CDK9 (Cyclin-dependent kinase 9), forming a complex that is critical for the regulation of transcriptional elongation by RNA polymerase II. Antibodies against CCNK are widely used in research focused on transcriptional regulation, cell cycle studies, and the role of CCNK-CDK9 complexes in various cellular processes.

Content on CCNK Antibodies

CCNK antibodies are highly specific and are primarily used in various applications such as Western blotting, immunohistochemistry, immunoprecipitation, and ELISA. These antibodies help in detecting the expression level of CCNK, providing insights into its regulatory mechanisms in the cell cycle, especially during the transition between transcriptional activation and elongation. Researchers also employ CCNK antibodies to study the phosphorylation events mediated by the CCNK-CDK9 complex, which are crucial for understanding diseases related to cell cycle dysregulation, such as cancer.

Applications and Importance

• Western Blotting: CCNK antibodies are used to detect protein levels in lysates derived from cells and tissues, providing valuable data on CCNK expression under different conditions.
• Immunohistochemistry: For tissue-specific localization of CCNK, these antibodies enable the visualization of cyclin K in formalin-fixed or paraffin-embedded tissues.
• Immunoprecipitation: This application allows for the study of protein-protein interactions, such as the binding of CCNK with CDK9, helping to elucidate the structural and functional relationships of the CCNK-CDK9 complex.
• ELISA: Quantitative analysis of CCNK levels is performed using antibodies in sandwich or competitive ELISA formats, offering precise data on cyclin K abundance in various biological samples.

Given the crucial role of CCNK in maintaining the transcriptional activity and its implication in disease pathology, antibodies targeting CCNK are indispensable tools in molecular biology, particularly for research in oncology and transcription-related disorders.