CBL Antibodies
CBL antibodies target the CBL (Casitas B-lineage lymphoma) protein, an E3 ubiquitin ligase that plays a crucial role in regulating signal transduction and receptor downregulation. CBL proteins (CBL, CBL-b, and CBL-c) mediate the ubiquitination and degradation of activated receptor tyrosine kinases (RTKs) and other signaling molecules, thereby controlling cell signaling pathways related to growth, differentiation, and apoptosis. Mutations in CBL are associated with several cancers and immune disorders due to their role in aberrant signaling regulation.
Application of CBL Antibodies
- Immunofluorescence (IF): In IF, CBL antibodies are used to visualize the subcellular localization of CBL in various cell types. This application is critical for studying how CBL regulates intracellular signaling by controlling the degradation of RTKs and other signaling proteins. Immunofluorescence with CBL antibodies provides insights into CBL’s role in processes like endocytosis, receptor recycling, and signal attenuation.
- Flow Cytometry (FACS): Fluorophore-conjugated CBL antibodies are used in flow cytometry to quantify CBL expression in specific cell populations. This application is valuable for studying CBL function in immune cells or cancer cell lines, where alterations in ubiquitin-mediated receptor degradation play key roles in disease pathogenesis.
- Co-Immunoprecipitation (Co-IP): CBL antibodies are used in Co-IP assays to investigate the interactions between CBL and other proteins involved in signaling pathways, such as RTKs or downstream effectors. This is essential for understanding how CBL regulates signal transduction by promoting the ubiquitination and degradation of its binding partners, helping dissect the molecular mechanisms behind receptor downregulation.
- Enzyme-Linked Immunosorbent Assay (ELISA): CBL antibodies are also used in ELISA to quantify CBL protein levels in biological samples, such as serum or cell extracts. This provides a useful tool for studying CBL expression in clinical samples and evaluating its potential as a biomarker for disease diagnosis or progression, particularly in cancer or immune disorders.
Technical Specifications
- Clonality: Monoclonal or Polyclonal
- Isotype: IgG (species-specific variants)
- Species Reactivity: Human, Mouse, Rat, and other species depending on the specific antibody
- Applications: WB, IHC, IF, FACS, Co-IP, ELISA
- Post-Translational Modification Detection: Available for detecting phosphorylated or ubiquitinated forms of CBL
- Storage Conditions: Typically stored at -20°C or -80°C to maintain long-term stability
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