CASP10 Antibodies

CASP10 antibodies are specific immunoglobulins designed to target Caspase-10 (CASP10), an initiator caspase that plays a vital role in the extrinsic apoptotic pathway. Caspase-10 is closely related to Caspase-8 and is activated upon stimulation by death receptors such as Fas (CD95) and TRAIL receptors. Upon activation, CASP10 initiates a cascade of events leading to apoptosis, which is essential for immune regulation, tissue homeostasis, and the elimination of damaged or cancerous cells. 

Content

Caspase-10 (CASP10) is a cysteine protease that becomes activated in response to extrinsic death signals. When death receptors such as Fas or TRAIL-R are engaged by their ligands, CASP10 is recruited to the death-inducing signaling complex (DISC) via adaptor proteins like FADD. At the DISC, CASP10 is cleaved and activated, leading to the subsequent activation of downstream effector caspases (e.g., caspase-3 and caspase-7) that execute apoptosis. Mutations or dysregulation in CASP10 can lead to impaired apoptotic signaling, contributing to immune-related disorders, autoimmune diseases, and resistance to cell death in cancers. 

Applications

• Immunohistochemistry (IHC): CASP10 antibodies are used in IHC to visualize the expression and localization of Caspase-10 in tissue sections. This application helps researchers study the spatial distribution of CASP10 in tissues, especially in cancer biopsies, where its expression might correlate with tumor progression or resistance to apoptosis.
• Flow Cytometry: Researchers use CASP10 antibodies in flow cytometric assays to analyze the expression of CASP10 at a single-cell level. This is particularly useful for examining the activation status of CASP10 in immune cells, cancer cells, or other cell populations exposed to apoptotic stimuli.
• Immunoprecipitation: CASP10 antibodies can be utilized to pull down Caspase-10 and its associated protein complexes from cell extracts, facilitating the study of protein-protein interactions within the apoptotic signaling cascade. This technique helps to uncover how CASP10 integrates signals from death receptors and initiates apoptosis.

CASP10 antibodies are essential tools in apoptosis research, allowing for detailed analysis of caspase activation and function. They provide insights into the mechanisms of programmed cell death and the regulation of the immune system, supporting the development of new therapeutic strategies for diseases characterized by abnormal apoptotic signaling, such as cancer, autoimmune disorders, and immunodeficiencies.