C(CST3) Antibodies
C (CST3) antibodies are specialized antibodies designed to target and detect cystatin C (CST3), a small cysteine protease inhibitor that plays a crucial role in regulating the activity of proteolytic enzymes, particularly cathepsins. Cystatin C is produced by nearly all nucleated cells and is present in various body fluids, including blood, cerebrospinal fluid (CSF), and urine.
Content: Cystatin C is considered a reliable biomarker for several physiological and pathological processes:
- Neurological Disorders: Cystatin C is involved in neuroprotection and has been studied for its role in neurodegenerative diseases, such as Alzheimer's disease. It helps inhibit proteases that degrade proteins involved in amyloid plaque formation, which is a hallmark of Alzheimer's. CST3 antibodies are used in research to study cystatin C’s distribution in the brain and its potential role in neurodegenerative conditions.
- Cardiovascular Diseases: Elevated levels of cystatin C have been associated with an increased risk of cardiovascular events, such as heart failure and atherosclerosis. CST3 antibodies are used to investigate the correlation between cystatin C and cardiovascular risk factors, helping to identify individuals at higher risk of cardiovascular diseases.
Applications: C (CST3) antibodies are widely used in various research and clinical applications, including:
- Western Blotting (WB): To detect and quantify cystatin C in cell lysates or tissue extracts. This technique is useful for studying the expression patterns of cystatin C in different tissues and under various conditions. Western blotting with CST3 antibodies can help analyze how cystatin C levels change in response to kidney injury, neurodegenerative processes, or cardiovascular conditions.
- Immunohistochemistry (IHC) and Immunofluorescence (IF): To visualize the localization and distribution of cystatin C in tissues. These techniques allow researchers to study the role of cystatin C in specific organs, such as the brain, kidney, and heart. For example, CST3 antibodies can be used to investigate the presence of cystatin C in brain plaques, contributing to research on Alzheimer’s disease.
- Flow Cytometry: To analyze the expression of cystatin C on the surface of cells or within cell populations. Flow cytometry with CST3 antibodies can be used to study the role of cystatin C in cell signaling and protease regulation, as well as to investigate how its expression changes under different experimental conditions.
C (CST3) antibodies are critical tools in biomedical research and clinical diagnostics. They enable the study of cystatin C’s role in regulating proteolysis, provide insights into its function in various organs, and help in the early detection and monitoring of diseases.
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