AURKB Antibodies

AURKB (Aurora Kinase B) antibodies target the Aurora Kinase B protein, a serine/threonine kinase crucial for regulating various stages of mitosis. Aurora Kinase B is part of the chromosomal passenger complex (CPC), which ensures accurate chromosome segregation and cytokinesis. It plays an essential role in mitotic events such as kinetochore-microtubule attachment, spindle checkpoint signaling, and the final separation of daughter cells. Dysregulation of AURKB is implicated in several cancers due to its role in controlling cell division.

Content and Structure of AURKB Antibodies

AURKB antibodies are available in monoclonal or polyclonal forms, generated against different epitopes of the AURKB protein. These antibodies are typically specific to the active (phosphorylated) or inactive form of Aurora Kinase B, depending on the application. Monoclonal antibodies are highly specific and are often used in assays requiring precise detection of AURKB. Polyclonal antibodies may offer broader epitope recognition, which can be useful in detecting the protein across different species or experimental conditions.

Application of AURKB Antibodies

• Western Blotting (WB): AURKB antibodies are extensively used in Western blotting to detect endogenous or overexpressed AURKB levels in cell lysates. Detection of phosphorylated AURKB is a key application, as it provides insights into the kinase's activation status during the cell cycle. 
• Immunohistochemistry (IHC): AURKB antibodies enable the detection of Aurora Kinase B in tissue sections, allowing researchers to visualize its expression pattern in normal and cancerous tissues. IHC staining can be used to study the localization of AURKB in mitotic cells, particularly in tumor samples where AURKB overexpression is a common oncogenic feature.
• Immunofluorescence (IF): In IF applications, AURKB antibodies help identify the precise subcellular localization of AURKB during mitosis. This is crucial for understanding the kinase’s role in chromosome segregation, spindle assembly, and cytokinesis. Dual staining with other cell cycle markers is often performed to track AURKB dynamics throughout mitosis.

Technical Specifications

• Clonality: Monoclonal or Polyclonal
• Isotype: IgG (varies by species)
• Species Reactivity: Human, Mouse, Rat, with potential cross-reactivity in other species
• Applications: WB, IHC, IF, ChIP, FACS, ELISA