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ARRB Antibodies

ARRB antibodies are developed to specifically target arrestin proteins, particularly Arrestin Beta 1 (ARRB1) and Arrestin Beta 2 (ARRB2), which are key regulators of G-protein-coupled receptor (GPCR) signaling. Arrestins are multifunctional proteins that play a central role in desensitizing GPCRs, terminating their signaling, and facilitating receptor internalization and recycling.

Content

Arrestins are a family of proteins that are crucial for the regulation of GPCRs, which are the largest class of cell surface receptors and are involved in numerous physiological processes, including vision, taste, and neurotransmission. When a GPCR is activated by a ligand (e.g., hormones, neurotransmitters), it triggers downstream signaling by coupling with G-proteins. Arrestins, specifically ARRB1 and ARRB2, bind to the phosphorylated form of the activated receptor, preventing further G-protein signaling.

Application

ARRB antibodies are utilized in various research and diagnostic applications, including:

Immunofluorescence (IF): Allows for high-resolution visualization of ARRB1 and ARRB2 at the cellular level, showing their specific localization within cells and interactions with other proteins. IF is particularly useful for studying receptor trafficking, internalization, and the role of arrestins in signaling networks.
Flow Cytometry: Applied to analyze ARRB protein expression on a per-cell basis, which can help quantify changes in arrestin levels in response to various stimuli or during disease progression. Flow cytometry is beneficial for studying the role of arrestins in immune cell signaling and cancer cell proliferation.
Immunoprecipitation (IP): Used to isolate ARRB proteins from complex protein mixtures, enabling the study of their interactions with GPCRs, kinases, and other signaling molecules. IP helps in understanding the molecular mechanisms through which ARRBs modulate receptor signaling and cellular responses.

These applications make ARRB antibodies critical tools for advancing research on GPCR signaling, receptor regulation, and cellular signaling networks. By studying ARRB proteins, researchers can better understand how GPCR signaling is controlled and how arrestins contribute to alternative signaling pathways beyond classical G-protein activation.