AMH Antibodies

AMH (Anti-Müllerian Hormone) antibodies are utilized in research and clinical diagnostics to detect and study Anti-Müllerian Hormone (AMH), a key regulator in reproductive and developmental biology. AMH is a glycoprotein hormone produced by the Sertoli cells in males and by the granulosa cells in females. It plays a crucial role in sexual differentiation during embryonic development and in regulating ovarian function in adults.

Structure and Function of AMH

• Structure:
  • Domains: AMH is a dimeric glycoprotein composed of two identical subunits linked by disulfide bonds.
  • Precursor: Synthesized as a prohormone, which is cleaved into the active form.
• Functions:
  • In Males:
    • Sexual Differentiation: AMH causes the regression of Müllerian ducts during fetal development, preventing the formation of female reproductive structures.
  • In Females:
    • Ovarian Reserve: AMH is produced by granulosa cells of preantral and small antral follicles; its levels reflect the number of remaining follicles (ovarian reserve).

Applications of AMH Antibodies

• Western Blotting:
  • Purpose: Detect and quantify AMH protein levels in cell or tissue extracts.
  • Application: Study AMH expression and analyze protein modifications.
• Immunohistochemistry (IHC):
  • Purpose: Localize AMH in tissue sections.
  • Application: Investigate AMH expression in ovarian and testicular tissues, and analyze changes in pathological conditions.
• Immunoassay (ELISA):
  • Purpose: Quantify AMH levels in serum or other biological fluids.
  • Application: Measure AMH levels for assessing ovarian reserve in females and diagnosing conditions such as polycystic ovary syndrome (PCOS) and premature ovarian failure.
• Immunoprecipitation (IP):
  • Purpose: Enrich AMH proteins from cell lysates.
  • Application: Study protein interactions and post-translational modifications.