TMPRSS Antibodies

TMPRSS (Transmembrane Serine Protease) belongs to a family of serine proteases that are anchored to the plasma membrane. These enzymes play critical roles in a variety of physiological processes including cell signaling, tissue remodeling, and viral entry. TMPRSS, particularly TMPRSS2, has gained significant attention for its role in facilitating the entry of respiratory viruses, such as SARS-CoV-2, into host cells by cleaving and activating viral spike proteins.

Technical Specifications

• Host Species: TMPRSS antibodies are commonly generated in hosts such as rabbits or mice, with recombinant monoclonal antibodies also available.
• Clonality: Both monoclonal and polyclonal antibodies exist. Monoclonal antibodies target specific epitopes of TMPRSS2, while polyclonal antibodies can recognize multiple epitopes, increasing sensitivity but reducing specificity.
• Isotypes: The isotypes of monoclonal TMPRSS2 antibodies are typically IgG, though other isotypes may be used based on the application.
• Applications: TMPRSS antibodies are widely used in:
  • Western blotting (WB): Detecting TMPRSS2 protein expression in cell lysates.
  • Immunohistochemistry (IHC): Visualizing TMPRSS2 in tissue sections.
  • Flow cytometry: Quantifying TMPRSS2 on the cell surface.
  • ELISA: Quantifying TMPRSS2 levels in biological fluids.
  • Immunoprecipitation (IP): Enriching TMPRSS2 protein from complex mixtures.

Molecular Applications of TMPRSS Antibodies

• Viral Entry Research: TMPRSS2 is a critical protease involved in the activation of viral spike proteins, such as those of coronaviruses, including SARS-CoV-2. Antibodies against TMPRSS2 can help elucidate the mechanisms of viral entry into host cells. These antibodies are particularly used in studies focused on the inhibition of TMPRSS2 to block viral infection.
• Cancer Research: TMPRSS2 is implicated in prostate cancer progression due to its role in androgen receptor signaling. TMPRSS2-ERG gene fusions are common in prostate cancer, making TMPRSS2 antibodies crucial for diagnostic and therapeutic research in oncology.
• Tissue Differentiation Studies: TMPRSS2 expression is observed in various epithelial tissues. TMPRSS antibodies are utilized to assess tissue-specific expression and differentiation markers, particularly in studies of the lung, prostate, and gastrointestinal tissues.

TMPRSS antibodies, particularly those targeting TMPRSS2, are essential tools in research areas ranging from viral pathogenesis to cancer biology. These antibodies are indispensable for studying proteolytic processes on the cell membrane, viral-host interactions, and potential therapeutic targets for diseases such as COVID-19 and prostate cancer.