PS727 Antibodies

PS727 antibodies are designed to target and recognize the phosphorylated serine residue at position 727 (pSer727) of specific proteins. Phosphorylation at Ser727 is a critical post-translational modification (PTM) that can modulate protein function, affecting various cellular processes such as signal transduction, transcriptional regulation, and cellular stress responses. 

Applications of PS727 Antibodies

• Western Blotting (WB): PS727 antibodies are employed to detect phosphorylated forms of proteins in cell lysates, allowing researchers to monitor the activation of signaling pathways. This application is critical in understanding the phosphorylation dynamics during cellular processes like growth factor signaling and cytokine responses.
• Immunohistochemistry (IHC): PS727 antibodies are used to visualize the localization and phosphorylation status of Ser727 in tissue sections, particularly in the context of disease progression such as cancer, where altered phosphorylation patterns are common.
• Immunofluorescence (IF): These antibodies can be used to stain and localize phosphorylated proteins within cells, enabling the visualization of dynamic phosphorylation events in response to stimuli, particularly in signaling pathways and nuclear-cytoplasmic shuttling of transcription factors like STAT3.
• Flow Cytometry (FC): In flow cytometry, PS727 antibodies help quantify the phosphorylation status of proteins across cell populations, which is particularly useful in studying immune cell activation or cancer cell signaling.

PS727 Antibodies in Disease Research

• Cancer: Aberrant phosphorylation of Ser727 in proteins like STAT3 is linked to oncogenesis. In many cancers, excessive activation of STAT3 contributes to tumor progression, immune evasion, and chemoresistance. PS727 antibodies enable the investigation of STAT3 activation status in cancer cells, providing insights into tumor biology and potential therapeutic targets.
• Inflammatory Diseases: STAT3 Ser727 phosphorylation plays a critical role in immune response regulation. PS727 antibodies are used to explore dysregulated STAT3 signaling in autoimmune and inflammatory conditions, contributing to understanding the molecular mechanisms driving these diseases.
• Cardiovascular Diseases: Ser727 phosphorylation in signaling proteins has been implicated in cardiac hypertrophy and heart failure. PS727 antibodies help in studying how altered phosphorylation affects cardiac function and may offer insights into therapeutic interventions.

PS727 antibodies are crucial tools for studying the phosphorylation-dependent regulation of protein function in various cellular processes. They are particularly valuable for investigating signal transduction pathways, transcriptional regulation, and cellular stress responses where Ser727 phosphorylation plays a key role.