NPM Antibodies

NPM (Nucleophosmin), also known as B23, is a multifunctional nucleolar phosphoprotein involved in various cellular processes, including ribosome biogenesis, centrosome duplication, cell cycle regulation, and stress responses. NPM is highly abundant in the nucleolus, where it interacts with ribosomal proteins and ribosomal RNA (rRNA), playing a crucial role in the assembly of ribosomes.

Applications of NPM Antibodies
  • Western Blotting: NPM antibodies are commonly used to detect NPM protein in cell lysates or tissue extracts. This application is crucial for studying NPM expression levels under different conditions, such as cell stress or cancer progression, and for identifying alterations in NPM protein structure or processing (e.g., in the case of the NPM1 mutation in AML).
  • Immunohistochemistry (IHC): NPM antibodies are applied to tissue sections to localize NPM in the nucleolus and cytoplasm. This technique is particularly useful in cancer diagnosis, especially in detecting the cytoplasmic accumulation of NPM in AML patients carrying the NPM1 mutation.
  • Immunocytochemistry (ICC): In cultured cells, NPM antibodies are used to visualize the subcellular localization of NPM, which is primarily nucleolar but can translocate to the cytoplasm in response to stress or oncogenic transformation. ICC studies help elucidate how NPM contributes to nucleolar function and its dynamic relocalization under different cellular conditions.
NPM Antibodies in Research

NPM antibodies are widely used in both basic and clinical research to explore the following areas:

  • Ribosome Biogenesis: NPM is a key regulator of ribosome assembly in the nucleolus. Researchers use NPM antibodies to study how NPM facilitates the interaction between ribosomal RNA and ribosomal proteins and how this process is affected by cellular stress or cancer.
  • Cell Cycle and Centrosome Duplication: NPM is involved in centrosome duplication and plays a critical role in ensuring proper cell division. NPM antibodies are employed to investigate how NPM regulates centrosome dynamics and how dysregulation of NPM contributes to genomic instability and tumorigenesis.
  • Cancer Diagnostics and Therapeutics: NPM antibodies are used in cancer diagnostics, particularly for identifying AML patients with NPM1 mutations. Additionally, researchers are exploring NPM as a therapeutic target, and NPM antibodies are being developed for potential use in targeted therapies that disrupt its oncogenic functions.

NPM antibodies are invaluable tools for studying the diverse functions of Nucleophosmin in cellular biology and disease. Their applications range from exploring ribosome biogenesis and cell cycle regulation to diagnosing and investigating the molecular mechanisms of cancer, particularly AML. 

AffiAB® NPM3 Polyclonal Antibody
CAT# AFG-PAB-10318
Size: 150 µL
Conjugation: Unconjugated
1,112.50 1112.5 USD
AffiAB® NPM2 Polyclonal Antibody
CAT# AFG-PAB-10317
Size: 100 µg
Conjugation: Unconjugated
533.50 533.5 USD
AffiAB® Phospho-NPM1 - Thr199 Polyclonal Antibody
CAT# AFG-PAB-10316
Size: 100ul
Conjugation: Unconjugated
625.00 625.0 USD
AffiAB® Phospho-NPM1 - T199 Polyclonal Antibody
CAT# AFG-PAB-10315
Size: 100 µg
Conjugation: Unconjugated
335.50 335.5 USD
AffiAB® Phospho-NPM1 - T234 Polyclonal Antibody
CAT# AFG-PAB-10314
Size: 100 µg
Conjugation: Unconjugated
335.50 335.5 USD
AffiAB® NPM1 Polyclonal Antibody
CAT# AFG-PAB-10313
Size: 100 µg
Conjugation: Unconjugated
335.50 335.5 USD
AffiAB® HNRNPM Polyclonal Antibody
CAT# AFG-PAB-06998
Size: 100 µg
Conjugation: Unconjugated
335.50 335.5 USD
AffiAB® CENPM Polyclonal Antibody
CAT# AFG-PAB-02587
Size: 100 µL
Conjugation: Unconjugated
533.50 533.5 USD
AffiAB® Anti-HNRNPM Antibody [JE64-62]
CAT# AFG-HB-1919
Size: 100 μL
496.50 496.5 USD
AffiAB® HNRNPM Antibody
CAT# AFG-IVA-13838
Size: 100 µg
Terminal: N/A
719.15 719.15 USD
AffiAB® CENPM Antibody
CAT# AFG-IVA-05935
Size: 25 µL
Terminal: Middle region
378.95 378.95 USD
AffiAB® CENPM Rabbit Polyclonal Antibody
CAT# AFG-EKA-10030
Size: 100 µL
246.00 246.0 USD
AffiAB® NPM Rabbit Polyclonal Antibody
CAT# AFG-EKA-02485
Size: 100 µL
246.00 246.0 USD