KLH Antibodies
KLH (Keyhole Limpet Hemocyanin) Antibodies are designed to target and bind to KLH, a large, copper-containing glycoprotein commonly used as a carrier protein in immunology and vaccine development. KLH is derived from the keyhole limpet (Megathura crenulata), a marine mollusk. It functions as an immune-stimulatory protein due to its high molecular weight and complex structure, making it highly immunogenic.
Content of KLH Antibodies
KLH Antibodies are generated by immunizing animals such as rabbits or mice with KLH or KLH-conjugated proteins. These antibodies can be monoclonal or polyclonal:
- Polyclonal KLH Antibodies recognize multiple epitopes across the KLH protein, providing robust detection and wide utility across applications.
- Monoclonal KLH Antibodies are specific to a single epitope on the KLH protein, ensuring high specificity in applications requiring precise detection.
Application of KLH Antibodies
KLH Antibodies are extensively used in various research fields, particularly in immunology and vaccine development. Common applications include:
- Vaccine Development: KLH is used as a carrier protein in conjugate vaccines to elicit a robust immune response to small antigens. KLH Antibodies are employed to monitor the immune response against the KLH carrier protein. By detecting the presence and magnitude of anti-KLH antibodies in serum, researchers can assess the efficacy of the immune response in vaccinated individuals or animals.
- Hapten-Carrier Systems: In immunological studies, small molecules (haptens) are often non-immunogenic on their own. KLH Antibodies are used to track the immune response to hapten-KLH conjugates. This is particularly important in the development of antibodies against small antigens like peptides, drugs, or environmental chemicals by coupling them to KLH to increase immunogenicity.
- Immunoassays (ELISA, Western Blot): KLH Antibodies are applied in immunoassays to detect KLH or KLH-conjugated antigens. In ELISA, KLH Antibodies can quantify KLH-conjugated antigens or antibodies against KLH in various samples, including serum, plasma, and cell culture supernatants. In Western blot assays, these antibodies can verify the presence of KLH or KLH-conjugated proteins in protein extracts or purified preparations.
KLH Antibodies are essential tools in immunological research and vaccine development, providing crucial insights into immune responses against KLH and KLH-conjugated antigens. Their applications span from basic research in immune function and vaccine efficacy to translational studies in cancer immunotherapy and immunotoxicology. By enabling the detection and quantification of KLH in various experimental systems, these antibodies are integral in advancing both fundamental and clinical research.
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